미생물학회지 (Korean Journal of Microbiology)

  • 제35권3호
  • /
  • Pages.173-179
  • /
  • 1999
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  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

각종 작물로부터 분리한 Rhizoctonia solani 균주의 RFLP 및 PCR-RFLP를 이용한 분자계통한 특성 구명

Molecular Systematics of Rhizoctonia solani Isolates from Various Crops with RFLP and PCR-RFLP

  • 최혜선 (강원대 농업생명과학대 식물응용과학부) ;
  • 신환성 (강원대 농업생명과학대 식물응용과학부) ;
  • 김희종 (강원대 농업생명과학대 식물응용과학부) ;
  • 김경수 (강원대 농업생명과학대 식물응용과학부) ;
  • 우수진 (강원대 농업생명과학대 식물응용과학부)
  • 발행 : 1999.09.01
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초록

Rhizoctonia solani 분류를 위해 균사의 anastomosis group과 grouprks의 유전적 차이의 분석을 위해 rDNA 의 PCR-RFLP 와 RFLP를 실시하였다. rDNA 의 PCR-RFLP 결과 R. solani 는 크게 5 group 으로 나뉘어졌다. 균주 번호 1번과 3번은 AG-5 그룹과 0.976의 높은 상동성을 보였고, 12번, 13번은 AG-2-2와 0.976의 상동성을 보였다. 균주번호 7,8,11,13,15 는 AG-1에 속하였다. 제한효소 HaeIII로 절단하였을 때 균주번호 4,5,7,8은 700 bp에서 하나의 밴드가 나타나 AG-1에 속하였고, 균주번호 9는 AG-2-1과 517 bp에서 하나의 밴드가 나타났다. 도한 제한효소 Msp I을 사용하여 southern blotting을 한 결과 더 다양한 절단양상을 보이며 AG 간의 차이가 나타났다. AG-2-1과 균주번호 9는 200 bp에서 하나의 밴드가 나타났다. 균주 3,4,5,10,11,13은 AG-1과 1kb에서 하나의 밴드로 나타났다.

As a result of PCR-RFLP, the isolates used in this study were classified into five groups. Isolates 1 and 3 were included in AG-5 with 97% genetic similarity. Isolates 12 and 13 were included in AG-1 wilh 100% genetic similarily. Isolates 10 and AG-2-2 showed 97% similarity Isolates 7, 8, 11. 13, and 15 were included in AG-1. When isolates of 4, 5, 7 and 8 were restricted with Hae I. there was a single 700 bp fragment matched with AG-1. A 517 bp restriction fragment of isolate 9 was matched with AG-2-1. Based on the result of southem hybridization of genomic DNAs, all isolates restricted with Msp I showed more variable restriction differences than those restricted with Hae Ill. Isolates AG-2-1 and 9 showed 200 bp restriction fragment, and isolates 3 and AG-1 showed 1 kb restriction fragments.

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