한국가금학회지 (Korean Journal of Poultry Science)

  • 제26권2호
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  • Pages.119-129
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  • 1999
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

외부유전자의 전이에 의한 배아세포와 트란스젠닉 가금 생산의 가능성

Possible Production of Transgenic Chicken by Transferring Foreign Genes and Germ Cells

  • Fujihara, N. (Laboratory of Animal Reproductive Physiology, Animal Resource Science Section, Division of Bioresource and Bioenvironmental Sciences, Graduate School)
  • 발행 : 1999.06.01
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초록

In recent years, numerous researches have been carried out in author's laboratory to develop several kinds of methods for producing transgened chicken, leaving a lot of new findings. Some of them are very useful to search for new approaches necessary to improve the efficiency of hatchability and the survival rate of developing trasgened embryos. The results obtained hitherto might be summarized as follows: (1) foreign gene(Lac Z/ Miw Z) introduced into blastodermal cells of developing embryos was successfully transferred to embryos, leading to the production of primordial germ cells(PGCs) carrying foreign DNA. However, hatched hickens failed to show the incorporation of introduced gene into the gonads. (2) When foreign gene was introduced into germinal crescent region (GCR), the gene was also efficiently incorporated into germ cells, resulting in the production of transgened chickens(offspring) which produced fruther offspring having foreign gene in the gonads. In this case, 2nd and 3rd generations of chickens were obtained through the reproduction of transgened birds. (3) In another way, the gene was injected into blood vessels of developing embryos at stage 13∼15, creating PGCs having foreign gene, and produced some transgened chickens. In this work, the PGCs were transfered between embryos, resulting in the production of transgenic chickens. (4) in these experiments, PGCs were effectively employed for producing transgenic birds, developing some kinds of chimeric chickens from homo- or hetero-sexual transfer of the PGCs from embryos. This means that the gonads from donor PGCs developed in some degree to the stage of hatching. However, these gonads showed slightly abnormal tissues similar to ovotestis like organs through histological examination. (5) Avian Leukosis Virus(ALV) induced B cell line(DT40) successfully carried foreign genes into chicken embryos, suggesting the possibility of the cells as a vector in this field of study in the future. (6) Inter-embryonic transfer of the PGCs also gave us some.

키워드

참고문헌

  1. Cell v.89 Reduced X-ray resistance and homologous recombination frequencies in a RAD54 metant of the chicken DT40 cell line Bezzubova O;Silbergleit A;Uamaguchi-Iwai Y;Takeda S;Buerstedde J-M
  2. EMBO J v.9 Light chain gene conversion continues at high rate in an ALV-induced cell line Buerstedde J-M;Reynaud C-A;Humphries EH;Olson W;Ewert DL;Weil J-C
  3. Cell v.67 Increased Ratio of targeted to random integration after trasfection of chicken B cell line Buerstedde J-M;Takeda S
  4. Green Fluorescent Protein Chalfie M;Kain S
  5. Proc 6th Asian Pacific Poult Cong Nagoya. Introduction fo exogenous genes into the gonads via primordial germ cells originated from germinal crescent of chicken embryos Ebara F;Hattori M;Fuhihara N
  6. Proc Int Cong Bird Reprod Tours In vivo gene transfer into chicken embryos via primordial germ cells using green fluorescene protein hybrid DNA Ebara F;Fujihara N
  7. Cytotechnol v.29 Introduction of exogenous genes into the gonada via primordial germ cells originated from germinal crescent of chicken embroys Ebara F;Fujihara N
  8. Asian J Androl v.1 Reproductive characteristics of transgenic(TG) chicken carrying exogenous genento Ebara F;Fujihara N
  9. Asian-Aust J Anim Sci. v.12 In vivo transfer of foreign DNA into primordial germ cells (PGCs)of chicken embryos Eguma k;Soh T;Hattori M;Fujihara N
  10. Dev Biol. v.49 From cleavage to primitive speak formation:a complementary normal table and a new look in the first stages of the development of the chick Eyal-Giladi H;Kochav S
  11. J Embryol Exp Morphol. v.65 Avian primodial germ cells are of epiblastic origin Eyal-Giladi H;Ginsburg M;Farbarov A
  12. Reproductive Biology Update In vivo gene transfer to chick embryos via spermatozoa and primordial germ cells Fujihara N;Inada S;Hasebe M;Yamaguchi H;Hattori M;Miyamoto H(ed);Manabe N(ed)
  13. Jpn Poult Sci. v.36 Poultry genetic resource and conservation biology-Review Fujihara N
  14. New Food Indust. v.41 Exploitation and utilization of genetic resources from wild and domesticated animals in the world Fujihara N
  15. Dev Biol. v.49 Observation of the primordial germ cells in blood samples from the stick embryo Fujimoto T;Ninomiya T;Ukeshima A
  16. Proc Int Conf Bird Reprod Tours Participation of embryonic germinal ridge storms cells(GRSCs) in in vitro proliferation of chicken primordial germ cells(PGCs) Fekushima R;Yang GQ;Fujihara N
  17. Proc 6th Asian Pacifica Poult Cong Nagoya. Gonadal development of chicken embryos derived firm hetero-sexual transfer of primordial germ cells Furuta H;Hattori M;Fujihara N
  18. Asian-Aust J Anim Sci. v.12 gonadal development of chicken embryos derived from hetero-sexual transfer of promordial germ cells Furuta H;Yamaguchi H;Fujihara N
  19. Proc Int Conf bird Reprod Tours Successful transfer of exogenous genes to chicken blastoderm by lipofection Furuta H;Kim KB;Fujihara N
  20. Poultry Scil. v.76 Primordial germ cell development in avians Ginsburg M
  21. Cellular and Molecular Biology of Gonadal Development and Maturation in Mammals Guraya SS
  22. J Morphol v.88 A series of normal stage in the development of chick embryo Hamburger V;Hamilton H
  23. Asian-Aust J Anim Sci. v.7 Gene transfer by manipulation of primordial germ cells in the chicken Han JY;Shoffner RN;Guise KS
  24. Asian-Aust J Anim Sci. v.7 Primordial germ cells in avain Han JY;Seo DS;Shoffner RN
  25. Proc AAAP Anim Sci Cong. v.2 the incorporarion of exogenes DNA into chicken spermatozoa Hasebe M;Okano K;Hattori M;Fujuhara N
  26. J Appl Anim Res. v.14 An attempt to produce transgenic chicken mediating sperm cells as vectors Haseba M;Soh T;Hattori M;Fujuhara N
  27. Asian-Aut J Anim Sci. v.8 Migration of the Primordial germ cells and gonadal formation in the early chicken embryo Hong YH;Seo DS;Jeong DK;Choi KD;Han JY
  28. Proc AAAP Anim Sci Cong. v.2 In vivo gene transfer into chick embryos at blastoderm stage of the oviposited eggs Inada S;Hanada K;Hattori M;Fujihara N;Morohashi K
  29. Reprod Nutr Dev. v.37 In vivo gene transfer into the blastoderm fo early developmental stage of chicken Inada S;Hattori M;Fujihara N;Morohashi K
  30. Proc JAACT/ESACT Transfection of foreign gene into chick embryos meditating blastodermal cells Inada S;Hattori M;Fujihara N
  31. Mol Reprod Dev. v.42 Sexual defferentiation of chimeric chickens containing ZZ and ZW cells in the germline Kagami H;Clerk ME;Gibbins AMV;Etches RJ
  32. World Poult Sci. v.53 Current knowledge of sexual differentiation in domestic fowl Kagami H;Hanada H
  33. Mol Reprod Dev. v.48 The developmental origin of primordial germ cells and the transmission of the donor-derived gamates imixed-sex germline chimeras to the offspring in the chicken Kagami H;Tagami T;Matsubara Y;Harumi T;Hanada H;Maruyama K;Sakurai M;Kuwana T;Naito M
  34. Develop Growth Differ. v.35 Migration of avain primordial germ cell toward the gonadal anlage Kuwana T
  35. Internat J Develop Biol. v.40 Long-term culture of avain embryonic cells in vitro Kuwana T;Hashimoto K;Nakanishi A;Yasuda Y;Tajima A;Naito M
  36. Cell v.57 Sperm cells as vectors for introducting foreign DNA into eggs:Genetic transformation of mice Lavitrano M;Camaioini A;Fazio VM;Dolci S;Ferrace MG;Spadafora C
  37. Br Poult Sci. v.38 Distribution of blastodermal cells transferred to chick embryos for chimera production using windowed eggs Marda T;Yamakawa Y;Masuda k;Terada T
  38. Poultry Sci.(abstr.) v.77 no.Suppl.1 Transfection of chicken blastoderm and experession of reporter constructs in early chicken embryos Maruyama K;Matsubara Y;Naito M
  39. Biochem Biophys Res Comm. v.230 Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo Meramatsu T;Mizutani Y;Omori Y;Okumara J
  40. Int J Mol Med. v.1 In vivo electroporation:A powerful and convenient means of nonviral gene transfer to tissues of living animals Meramatsu T;Nakamura A;Hyi-man P
  41. Int J Dev Biol. v.35 Embryonic experession of β-actin-lac Z hybrid gene injected into the fertilized ovum of the domestic fowl Naito M;Agata K;Otsuka K;Kino M Ohta M;Hirose K;Perry MM;Eguchi G
  42. Mol Reprod Dev. v.37 Introduction of exogenous DNA into somastic and germ cells of chicken by microinjeciton into the germinal disc of fertilized ova Naito M;Sasaki E;Ohtaki M;Sakurai M
  43. J Reprod Fert v.113 Expression of exogenes DNA in the gonads of chimaeric chicken embryos produced by transfer of primordial germ cells transfected in vitro and subsequent fate of the introduced DNA Naito M;Sakurai M;Kuwana T
  44. Mol Reprod Dev. v.36 Gene transfer in the chicken by spermmediated methods Nakanishi A;Irtani A
  45. Rrimordial Germ Cells in the Chordates Nieuwkoop PD;Sutasurya LA
  46. J Reprod Dev. v.41 Gene expression in blastocysts following direct injection of DNA into testies Ogawa S;Hayashi K;Tada N;Sato M;Kurihara T;Iwaya M
  47. Anim Sci Technol. v.69 Settlement of quail primordial germ cells in chicken gonads Ono T;Yokoi R;Maeda S;Nishida T;Aoyama H
  48. Development v.108 Production of somatic and germline chimeras in the chicken by transfer of early blastodermal cells Petitte JN;Clerk ME;Liu G;Verrinder Gibbins AM;Ettches RJ
  49. Poultry Sci. v.76 The origin of the avian germ line and transgenesis in birds Peitte JN;Karagenc L;Ginsburg M
  50. Methods in Dell Biology v.76 Green Fluorescent Proteins Sulliva KF;Kay SA
  51. Develop Growth Differ. v.39 Defferentiattion of female chicken primordial germ cells into spermatozoa in male gonads Tagami T;Matsubara Y;Hanada H;Naito M
  52. Proc Ann Meet Jpn Soc Zootech Sci.(abstr.) Transfer of foreign gene into chiken by sperm vector via lipofectamine Tanigawa M;Minami N;Yamada M;utsumi K
  53. Proc Int Conf Bird Reprod Tours Possible vector of DT40 cells for transferring foreign genes to chicken embryos Toba M;Ebara F;Fruta H;Matsushima Y;Kitagawa Y;Fujihara N
  54. Proc AAAP Anim Sci Cong. v.2 Effects of transfer of promordial germ cells(PGCs) into embryonic blood vessels on the exual character of chicks Yamaguchi H;Okano K;hattori M;Fujihara N
  55. Cytotechnol v.26 Inter-embryonic(homo-and heter-sexual) transfer of primodial ger cells(PGCs) between chicken emryos Yamagudchi H;Hattori M;Fujihara N
  56. Proc 6th Asian Pacific Poult Cong Nagoya Isolation and in votro culture of chicken PGCs Yang GQ;Fujihara N
  57. J Reprod Dev. v.45 Long-term proliferarion of chicken primordial germ cells cultured in vitro Yang GQ;Fujihara N
  58. J Reprod Dev. v.45 Survival and proliferarion of refined chicken circulating primordial germ cells cultured in vitro Yang GQ;Fujihara N