YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Cytotoxicity of Artemisia argyi Extract Against H9 (ATCC HTB 176) Cell and Antioxidant Enzyme Activities

황해쑥(Artemisia argyi)의 H9 (ATCC HTB176) 세포에 대한 세포독성 및 항산화효소 활성

  • 김경하 (상명대학교 자연과학부 화학과) ;
  • 정대영 (상명대학교 자연과학부 화학과) ;
  • 민태진 (동국대학교 자연과학부 화학과) ;
  • 박시원 (상명대학교 자연과학부 화학과)
  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The hot water and mathanol extracts of Artemisia argyi showed considerable cytotoxicities against H9(ATCC HTB 176) cancer cell with IC50 values of $48.6{\;}\mu\textrm{g}/ml$ and $51.9{\;}\mu\textrm{g}/ml$, respectively. These cytotoxicities were found to be dependent on the extract concentrations and culture days. CuZnSOD and MnSOD activities were significantly increased in the cytoplasm and mitochondria fractions of cancer cell, and media in the presence of Artemisia argyi. Such enhanced SOD activities were generally in the range of two to threefolds. In contrast to SOD, catalase and glutathione peroxidase activities were not detected at all. These results suggest that Artemisia argyi have generated $O_2^-$ in the mitochondria and cytoplasm of H9 cancer cell with concurrent induction of CuZnSOD and MnSOD in situ, which dismutate $O_2^-{\}to{\;}H_2O_2$. Without coordinated actions of catalase and/or glutathione peroxidase $H_2O_2$ is easily converted to very toxic OH and these reactive oxygen species together might have induced necrosis and/or apoptosis of H9 cell.

Keywords

References

  1. 鄕藥 大事典 鄭善變;辛民敎
  2. 韓國藥品 食品 貧源 圖鑑 陸昌壽
  3. Kor. J. Pharmacogn v.27 Antimutagenic Effect of Some Artemisia Species Park, K. Y.;Ha, J. O.;Yu, J. B.;Lee, J. H.;Park, J. C
  4. J. Pharmac. Sci. v.68 no.7 Antibacterial Artemisia herba Yashipe, J.;Segal, R.;Breuer, A;Naftali, G.
  5. Int. J. Immuno-pharmacol v.11 Immunological mechanisms of antitumor activity of some kinds of crude drugs on tumor necrosis factor production Xu, Q.;Mori, H.;Sakamoto, O.;Uesugi, Y.;Koda, A
  6. J. Pharmacol (JPN) v.49 Mechanisma of antitumor activity of aqueous extracts from Chinese herbs, and their immunological properties Mori, H.;Xu, Q.;Sakamoto, O.;Uesusi, Y.;Koda, A.;Nishioka, I
  7. Cancer J. Clin v.46 Cancer Statistics Parker, S. L.;Tong, T.;Bolder, S.;Winggo, P. A
  8. N. Eng. J. Med v.336 Cancer undefeated Bailer, J. C.;Gorrick, H. L
  9. The LANCET v.343 Rethinking Cancer Astrow, A. B.
  10. Carcinogenesis v.20 Environmental factors as regulators and effectors of multistep carcinogenesis Minamoto, T.;Mai, M.;Ronai, Z
  11. The LANCET v.344 Oxyradicals and cancer Cerutti, P. A.
  12. Free Radicals and Oxdation Phenomena in Biological Systems Definitions, properties and reactions of radicals Roberbroid, M.;Calderon, P. B.
  13. J. Pineal Res v.14 melatonin, hydroxy radical mediated oxidative damage and aging Poegelle, B.;Reiter, R.J.;Tan, D. X.;Chen, L. D.;Manchester, L. C.
  14. Prog. Lipid. Res v.32 Free radical lipid interactions and their pathological consequences Rice-Evance, C.;Burdon, R
  15. Toxicol. Indust . Health v.9 Tissue injury by free radicals Chessman, K. H.
  16. The FASEB J. v.9 Oxidative process and antioxidative defense mechanism in the aging brain Reitter, R
  17. The FASEB J v.10 Antioxidant and redox regulation of gene transctiption Sen, C. K.;Packer, L.
  18. Oxidative stress, gene expression and the aging process Guyton, K. Z.;Gorospe, M.;Holbrook, N. J.
  19. Free Radical Biology and Medicine v.23 Oxidative stress hypothesis in Alzheimer Disease Markesbery, W. R.
  20. Environmental Health Perpectives v.106 The role of oxidative stress in chemical carcinogenesis Klaunig,J.E.;Xu,Y.;Isenberg,J.S.;Bachowski,S.;Kolaja, K. L.
  21. Tips v.13 Cell death by apoptosis and its protective role against disease Bursch, W.;Oberhammer, F.;Schulte-Hermann, R
  22. Annals of Pharmacother v.31 Apoptosis: its role in the develpment of malignancies and its potential Dixon, S.;Soriano, B.J.;Lush, R. M.Bommer, M. M.;Figg, W. D.
  23. Cancer Chemotherpy Report Cytotoxicity assays with L 1210 cell in vivo and KB cells in vitro Thayer, P. S.;Himmelfarb, P.;Watts, G. L.
  24. Cancer Chemother.Rep v.3 Cell culture screen, KB. Protocol 1600 National Cancer Institute USA
  25. J. Biol. Chem. v.244 Superoxide dismutase (SOD) assay McCord, J. M.;Fridovich, I.
  26. Free Radical Biol. Med. v.15 Lopez-Torres, M.;Perex-Campo, R.;Rojas, C.;Cadens, S.;Barja, G
  27. Int. J. Cancer v.51 SOD 2:a new type of tumor suppressor gene? Bravard, A.;Sabatier, L.;Hofschir, F.;Ricoul, M.;Luccioni, C.;Dutrilaux, B
  28. J. Immunol v.147 Manganese superoxide dismutase is induced by IFN-γ in multiple cell types Harris, C. A.;Derbin, K. S.;Hunte-McDonough, B.;Epstein, L. B.
  29. J. Biol. Chem. v.244 Superoxide dismutase: an enzymic function of erythrocuprein(hemocuprein) McCord, J. M.;Fridovich, I
  30. The FASEB J v.9 Oxidative process and antioxidative defense mechanisms in the aging brain Reiter, R. J.
  31. Mayo Clin. Proc v.63 Free radicals in medicine Southern, P. A.;Powis, G
  32. Free Radicals and Oxidative Phenomena in biological Systems Antioxidants and radical scavengers: some therapeutical uses Roberfroid, M.;Calderon, P.B.
  33. J. Neurolog. Sci. v.122 Histochemical localization of glutathione peroxidase in infarcted human brain Takizawa, S.;Matsushima, K.;Shinohara, Y.;Ogawa, S.;Kormatsu, N.;Utsunomiya, H.;Watanabe, K.
  34. Oxidative processes and antioxidants Selenium-dependent peroxidase Urshini, F.;Paolletti, R.;Samuelson, B.;Cataparro, A. L.;Poli, A.;Rinnetti, N. (eds.)