Anti-allergic Actions of the Leaves of Castnea crenata and Isolation of an Active Component Responsible for the Inhibition of Mast Cell Degranulation

  • Lee, Eun (Pharmacology Laboratory, College of Pharmacy, Chonnam National University) ;
  • Choi, Eun-Ju (Korea Research Institute of Chemical Technology) ;
  • Cheong, Ho (Pharmacology Laboratory, College of Pharmacy, Chonnam National University) ;
  • Kim, Young-Ran (Pharmacology Laboratory, College of Pharmacy, Chonnam National University) ;
  • Ryu, Shi-Yong (Korea Research Institute of Chemical Technology) ;
  • Kim, Kyeong-Man (Pharmacology Laboratory, College of Pharmacy, Chonnam National University)
  • Published : 1999.06.01

Abstract

The anti-allergic actions of the leaves of Castanea crenata (Fagaceae) were studied. The water extract demonstrated potent anti-allergic actions in in vivo and in vitro experiments. The oral or intraperitoneal administration of the extract (100 or 200 mg/kg) caused a significant inhibition of the 48 hr-PCA (up to 90%) and the vascular permeability induced by histamine or serotonin in rats (about 80%). The anaphylactic release of ${\beta}$-hexosaminidase for RBL-2H3 cells was also significantly inhibited by the extract in as dose-dependent manner with an IC50 value of 230 $\mu\textrm{g}$/ml. The activity-guided fractionation of the extract, based on the determination of inhibitor effect upon the release of ${\beta}$-hexosaminidase, led to the isolation of quercetin as an active principle responsible for the inhibition of degranulation.

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