Archives of Pharmacal Research
- 제22권1호
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- Pages.13-17
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- 1999
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
The Kinetic Characteristics of K228G Mutant Horse Liver Alcohol Dehydrogenase
- Cho, Sun-Hyoung (College of Pharmacy, Ewha Womans University) ;
- Ryu, Ji-Won (College of Pharmacy, Ewha Womans University) ;
- Lee, Kang-Man (College of Pharmacy, Ewha Womans University)
- 발행 : 1999.02.01
초록
The kinetic constants and the reaction mechanism of the K228G mutant horse liver alcohol dehyrogenase isoenzyme E (HLADH-E) were compared to the wild-type enzyme. All the Km and Ki constants of the mutant enzyme for NAD+, ethanol, acetaldehyde and NADH were larger than those of the wild-type enzyme. The dissociation constants for the NADH and