BMB Reports
- 제32권6호
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- Pages.561-566
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- 1999
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- 1976-670X(eISSN)
Effects of the Hinge Region of Cecropin A(1-8)-Melittin 2(1-12), a Synthetic Antimicrobial Peptide on Antibacterial, Antitumor, and Vesicle-Disrupting Activity
- Shin, Song-Yub (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Kang, Joo-Hyun (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Jang, So-Yun (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Kim, KiI-Lyong (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Hahm, Kyung-Soo (Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology)
- 발행 : 1999.11.30
초록
CA(1-8)-ME(1-12) [CA-ME], composed of cecropin A(1-8) and melittin(1-12), is a synthetic antimicrobial peptide having potent antibacterial and antitumor activities with minimal hemolytic activity. In order to investigate the effects of the flexible hinge sequence, Gly-Ile-Gly, of CA-ME on antibiotic activity, CA-ME and three analogues, CA-ME1, CA-ME2, and CA-ME3, were synthesized. The Gly-Ile-Gly sequence of Ca-ME was deleted in CA-ME1 and replaced with Pro and Gly-Pro-Gly in CA-ME2 and CA-ME3, respectively. CA-ME1 and CA-ME3 showed a significant decrease in antitumor activity and phospholipid vesicle-disrupting ability. However, CA-ME2 showed similar antitumor and vesicle-disrupting activities, as compared with CA-ME. These results suggest that the flexibility or
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