BMB Reports
- 제32권1호
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- Pages.6-11
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- 1999
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- 1976-670X(eISSN)
Characterization of Phospholipid and Fatty Acid Composition in the Amp 1-4 Mutant Compared to Wild-Type Arabidopsis thaliana
- Nam, Im-Sook (Department of Biochemistry and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
- Hong, Yong-Geun (Department of Biochemistry and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
- Hwang, In-Hwan (Department of Biochemistry and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
- Cho, Moo-Je (Department of Biochemistry and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
- Pak, Yun-Bae (Department of Biochemistry and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
- 발행 : 1999.01.31
초록
To understand the function of phospholipids and their fatty acid composition on the morphological changes in the amp 1-4 mutant of Arabidopsis, the mutant was compared to the wild-type Arabidopsis by TLC, HPTLC, phosphorous assay, HPLC, and GC. In the mutant, phosphatidylethanolamine (PE) was increased 5-fold and phosphatidylglycerol (PG) was decreased 1.2-fold (nmol phosphorous/g tissue). Inositol phospholipids showed a generally increased trend ranging from 1.4-to 3.0-fold (nmol inositol/g tissue). When fatty acid composition of the mutant was compared to the wild-type, linoleic (18:2) and linolenic (18:3) acids of phosphatidylcholine (PC) and PG were decreased but palmitoleic acid (16:1) and oleic acid (18:1) of PC was increased 2.5- and 2.1-fold (mol%), respectively. In galactolipids, myristic acid (14:0) of monogalactosyl-diacylglycerol (MGDG) were increased 5.8-fold (mol%). Among the inositol phospholipids, lysophosphatidylinositol (L-PI) and phosphatidylinositol 4,5-bisphosphate (