원지(遠志)에 의한 뇌(腦) 성장세포(星狀細胞)로부터 염증성(炎症性) 세포활성물질(細胞活性物質) 분필(分泌)의 억제(抑制) 효과(效果)에 관(關)한 연구(硏究)

Studies on Inhibitory Effect of Inflammatory Cytokines Secretion from Brain Astrocytes by Polygala Tenuifolia

  • 황시영 (원광대학교 한의과대학 신경정신과 교실) ;
  • 강형원 (원광대학교 한의과대학 신경정신과 교실) ;
  • 류영수 (원광대학교 한의과대학 신경정신과 교실)
  • Hwang Si-Young (Dept. of Oriental Neuropsychiatry, College of Oriental Medicine, Won Kwang University) ;
  • Gang Hyeong-Won (Dept. of Oriental Neuropsychiatry, College of Oriental Medicine, Won Kwang University) ;
  • Lyu Yeong-Su (Dept. of Oriental Neuropsychiatry, College of Oriental Medicine, Won Kwang University)
  • 발행 : 1999.08.30

초록

We investigated whether an aqueous extract of Polygala tenuifolia root (PTAE) inhibits secretion of inflammatory cytokines from primary cultures of mouse astrocytes. PTAE dose-dependently inhibited the Tumor necrosis $factor-{\alpha}$ $(TNF-{\alpha})$ secretion by astrocytes stimulated with substance P (SP) and lipopolysaccharide (LPS). Interleukin-1 (IL-1) has been shown to elevate $TNF-{\alpha}$ secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore also investigated whether IL-1 mediated inhibition of $TNF-{\alpha}$ secretion from primary astrocytes by PTAE. Treatment of PTAE to astrocytes stimulated with both LPS and SP decreased IL-1 secretion to the level observed with LPS alone. Moreover, incubation of astrocytes with IL-1 antibody abolished the synergistic cooperative effect of LPS and SP. Reverse transcriptase-polymerase chain reaction analysis demonstrated the significantly reduced level of the $TNF-{\alpha}$ mRNA was expressed in astrocytes treated with PTAE. These results suggest that PTAE has an antiinflammatory activity on the central nervous system curing some pathological disease states.

키워드

참고문헌

  1. 圓光大學校 大學院 碩士論文 天門冬에 의한 腦神經膠細胞로부터 炎症性細胞活性物質 分泌의 抑制效果 강형원
  2. 增補萬病回春 공廷賢
  3. 大韓鍼灸學會誌 v.14 no.2 痴매治療의 最近 硏究動向에 關한 考察 金利和(外)
  4. 東醫神經精神科學會誌 v.8 no.2 Alzheimer형 痴매患者 2例에 對한 臨床的 考察 金保경(外)
  5. 대한한방내과학회지 v.18 no.2 痴매에 대한 文獻的 考察 김영균(외)
  6. 大韓韓醫學會誌 v.13 no.2 老人星 痴매에 關한 體質醫學的 硏究 裵旿星
  7. 大韓鍼灸學會誌 v.14 no.1 痴매에 對한 東西醫學의 文獻的 考察 徐政烈(外)
  8. 大韓韓醫學會誌 v.16 no.1 腦의 機能에 對한 臟象論的 考察 成彊慶
  9. 備急千急要方(卷四十) 孫思邈
  10. 東醫神經精神科學會誌 v.7 no.1 記憶障碍에 關한 東$\cdot$西醫學的 比較, 硏究 柳泳秀(外)
  11. 大韓神經科學會誌 v.3 no.1 精神科 영역에서의 痴매 이근후
  12. 大韓韓方內科學會誌 v.16 no.1 痴매에 關한 東西醫學的 比較 考察 李東垣(外)
  13. 編註醫學入門(卷二) v.2 李挻
  14. 大韓本草學會誌 v.12 no.1 遠志의 安神作用에 對한 藥理的 解析의 硏究 李체熙
  15. 東醫神經精神科學會誌 v.7 no.1 痴매에 對한 文獻的 考察 鄭仁哲(外)
  16. 惠和醫學 v.7 no.1 痴매患者 17例에 對한 臨床的 考察 鄭仁哲(外)
  17. 辨證錄 陣士鐸
  18. 大韓韓醫學會誌 v.17 no.1 健忘의 辨證分型에 對한 硏究 崔龍埈
  19. 古今醫鑑 공信
  20. 老年期痴매の對應 大友英一
  21. 中醫形神病學 董蓮榮等(編著)
  22. 中草藥學 上海中醫學院(編)
  23. 補賢活血化痰法治療老年性痴매 32例 v.29 no.5 徐恒旺
  24. 湖北中醫雜誌 v.18 no.124 益氣聰明湯治療腦血管神經性病變的체會 王乃石
  25. 中醫老年病學 袁立人(外)
  26. 中醫精神醫學 李淸福;劉渡舟(編著)
  27. 上海中醫藥雜誌 v.3 매從痰治 張覺人
  28. 北京中醫學大學學報 v.19 no.6 略論張錫純心腦共主神明說 程如海
  29. 本草求眞 黃宮繡(纂)
  30. 腦 金基錫(譯);Richard F. Thompson(著)
  31. 치매, 알츠하이머병 김승업
  32. 東醫生理學 金完熙(外編著)
  33. 標準本草學 金晸壽
  34. 大韓神經科學會誌 v.3 no.1 Alzheimer's disease의 신경화학적 변화에 관한 고찰 김진수
  35. 神經精神醫學 대한신경정신의학회
  36. 東醫病理學 文濬典(外)
  37. 成均館大學校 大學院 博士學位論文 遠志 Saponin의 利尿效果 및 中樞抑制 作用에 關한 硏究 朴大圭
  38. 臨床生理學 朴貞五(外)
  39. 老人醫學 배영철(外)
  40. 圓光大學校 大學院 博士論文 聰明湯이 腦組織의 生化學的 變化와 神經細胞의 損傷에 미치는 實驗的 硏究 徐敏華
  41. 東醫神經精神科學會誌 v.8 no.2 七福飮이 老化 白鼠 腦組織의 生化學的 變化에 미치는 影響 손정석
  42. 原色臨床本草學 辛民敎
  43. 本草備要解析 楊東喜
  44. 黃帝內經譯解(素問) 楊維傑(編)
  45. 黃帝內經譯解(靈樞) 楊維傑(編)
  46. 東醫神經精神科學會誌 v.8 no.1 調胃升淸湯이 흰쥐의 방사형 미로 학습과 기억에 미치는 영향 우주영
  47. 東醫生理學講義 柳道坤
  48. 노인의학 의학교육연구원
  49. 東醫心系內科學(上) 李京燮(外)
  50. 최신임상정신의학 이근후
  51. 大韓韓醫學會誌 v.16 no.2 麝香이 생쥐의 腦損傷에 미치는 影響 李保英;姜錫峯
  52. 韓藥臨床應用 李尙仁(外)
  53. 本草鋼目 李時珍
  54. 大韓韓醫學會誌 v.4 no.2 內經에 나타난 腦의 考察 이원철(外)
  55. 醫宗必讀 李中梓
  56. 張氏景岳全書 張介賓
  57. 辯證奇門全書 錢鏡湖
  58. 本草學 全國韓醫科大學 本草學敎材共(編著)
  59. 大韓神經科學會誌 v.3 no.1 치매(Dementia)의 병리 지제근
  60. 國譯石室秘錄 陣士鐸
  61. 圓光大學校 大學院 博士論文 定志丸이 腦組織의 生化學的 變化와 神經細胞의 損傷에 미치는 實驗的 硏究 崔龍埈
  62. 東醫神經醫學 黃義完(外)
  63. 東醫神經精神科學會誌 v.7 no.1 치매에 대한 한의학적 임상연구 黃義完(外)
  64. 中醫雜誌 v.38 no.10 謝海洲治療腦萎縮經驗 郭字鵬(外)
  65. 北京中醫學學報 v.19 no.6 謙齋醫學辨證論治的三位一體觀 孫其新;孫其然
  66. 中醫臨床大全 楊思澎(外)
  67. 遙寧中醫雜誌 v.23 no.3 中醫治療腦萎縮近況 楊連合
  68. 中醫函授通訊 v.16 no.2 論腦爲元神之府 王彩霞
  69. 醫林改錯 王淸任
  70. 本草品彙精要 劉文泰(等纂)
  71. 心-腦-神志病辨證論治 張明准(外)
  72. 中醫雜誌 v.35 no.3 老人性 腦病의 中醫診斷治療 傅仁杰(外)
  73. 福建中醫藥 v.25 no.1 腦萎縮的中醫康復治療 洪亨炤
  74. 實用中醫內科學 黃大東(外)
  75. J. Neuroimmunol. v.30 Induction and regulation of interleukin-6 gene expression in rat astrocytes Benveniste, E. N.;Saparcio, S. M.;Norris, J. G.;Grenett, H. E.;Fuller, G. M.
  76. J. Neuroimmunol. v.36 Interleukin-1 beta induction of tumor necrosis factoralpha gene expression in human astroglioma cells Bethea, J. R.;Chung, I. Y.;Sparacio, S. M. Gillespie, G. Y.;Benvensite, E. N.
  77. J. Neuroimmunol. v.18 Hypothesis: a role for tumor necrosis factor in immune-related admyelination and its relevance to myltiple sclerosis Brosnan, C. F.;Selmaj, F. K.;Raine, C. S.
  78. J. Neurochem. v.56 Regulation by interleukin-1 of nerve growth factor secretion and nerve growth factor mRNA expression in rat primary astroglial cultures Caman-Krzan, M.;Vige, X. ;Wise, B. C.
  79. Neuroscience Lett. v.129 Elevated circulationg tumor necrosis factor levels in Alzheimer's disease Fillit, H.;Ding;W. H.;Buce, L.;Kalman, J.;Altstiel, L.;Lawlor, B.;Wolf-Klein, G.
  80. Immunol. Rev. v.100 Immune-mediated encephalitis; on the role of antigen- presenting cells in brain tissue Fontana, A.;Frei, K.;Bodmer, S.;Hofer, E.
  81. J. Immunol. v.129 Production of prostaglandin E and an interleukin-1 like factor by cultured astrocytes and C-6 glioma cells Fontana, A.;Kristensen, F.;Dubs, R.;Gemsa, D.;Webew, E.
  82. Brain Res. (Mol. Brain Res.) v.16 Expression of amyloid precursor protein mRNAs in endothelial. neuronal. and glial cells: modulation by interleukin-1 Forloni, G.;Demicheli, F.;Giorgi, S.;Bendotti, C.;Angeretti, N.
  83. Proc. Natl. Acad. Sci. USA v.88 Cytokine regulation of substance P expression in sympathetic neurons Freidin, M.;Kessler, J. A.
  84. Proc. Natl. Acad. Sci. USA v.86 Brain interleukin-1 and S-100 immunoreactivity are elevated in Down syndrome and Alzhimer's disease Giffin, W. S.;Stanley, L. C.;Lung, C.;White, L.;MacLeod, V.;Perott, L. J.;White, C. L.;Araoz, C.
  85. j. Neurosci. v.8 interleukin-1 injection into mammalian brain stimulates astrogliosis and neovascularization Giulian, D. J.;Woodward, D. G.;Young, J. F.;Krebs;Lachman, L. B.
  86. Int. Arch. Allergy Immunol. v.105 Differential expression of protein kinase C genes in cultured mast cells derived from normal and mast-cell-deficient mice and mast cell lines Kim, H. M.;Hirota, S.;Chung, H. T.;Ohno, S.;Osada, S. I.;Shin, T.;Ko, K. I.;Kim, J. B.;Kitamura, Y.;Nomura, S.
  87. Science v.233 Detection of AIDS virus in macrophages in brain tissue form AIDS patients with encephalopathy Koening, S.;Gendelman, H.E.;Orenstein, J.M.;Dal Canto, M. C.;Pezeshkpour, G. H.;Yungbluth, M;Janotla, F.;Aksamit, A.;Martin, M. A.;Fauci, A. S.
  88. Scand. J. Immounol. v.31 The neuropeptide substance P stimulates production of interleukin 1 in human blood monocytes: activated cells are preferentially influenced by the neuropeptide Laurenzi, M. A.;Persson, M. A. A.;Dalsgaard, C. J.;Haegerstrand, A.
  89. Naunym-Schmie-ddeberg's Arch. Pharmacol. v.310 Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation Lembeck, F.;Holzer, P.
  90. Neuroscience v.3 Distribution of substance P-like immunoreactivity in the central nervous system of the rat-I. Cell bodies and nerve terminals Ljungdahl, A.;Hokfelt, T.;Nilsson, G.
  91. Science v.241 Effect of neuropeptides on production of inflammatory cytokines by human monocytes Lotz, M.;Vaughan, J. H.;Carson, D. A.
  92. J. Immunol. v.152 Substance P enhances the secretion of tumor necrosis factor-$\alpha$ from neuroglial cells stimulated with lipopolysaccharide Luber-Narod, J.;Kage, R.;Leeman, S. E.
  93. J. Immmunol. v.144 Production of hemopoietic colony-stimulation factors by astrocytes Malipiero, U. V.;Frei, K.;Fontana, A.
  94. Proc. Natl. Acad. Sci. USA v.86 Substance P receptor binding sites are expressed by glia in vivo after neuronal injury Mantyh, P. W.;Johnson D. J.;Boehmer, C. G.;Catton, M. D.;Vinters, H. V.;Maggio, J. E.;Too, H.-P.;Vigna, S. R.
  95. J. Neurol. v.239 Cerebrospinal fluid cytokines in AIDS demetia complex Perrella, O.;Carrieir, P. B.;Guarmaccia, D.;Soscia. M.
  96. Drug Devel. Res. v.15 Immune actions in the nervous system: a brief review with special emphasis on Alzheimer's Disease Rogers, J.;Luber-Narod, J.
  97. Lancet v.2 Raised serum levels of tumor necrosis factor in parasitic infentions Scuderi, P.;Sterling, K. E.;Lam, K. S.;Finley, P. R;Ryan, K. J.;Ray, C. G.;Petersen, E.;Slymen, D. J.;Salmon, S. E.
  98. Ann. Neurol. v.23 Tumor necrosis factor mediates myelin and oligodendrocyte damage in vitro Selmaj, K. W.;Raine, C. S.
  99. J. Immunol. v.144 Proliferation of astrocytes in vitro in response to cytokines: a primary role for tumor necrosis factor Selmaj, K. W.;Farooq, M.;Norton, W. T.;Raine, C. S.;Brosnon, C. F.
  100. J. Neurochem. v.51 TNF-induced proliferation of astrocytes from mature brains is associated with down-regulation of GFAP mRNA. Selmaj, K.;Shafit-Zagardo, B.;Aquino, D.;Farooq, M.;Raine, C. S.;Norton, W. T.;Brosnan, C. F.
  101. J. Neuroimmunol. v.38 In vivo relationship of tumor necrosis factor-$\alpha$ to blood-brain barrier damage in patients with active multiple sclerosis Sharief, M. K.;Thompson, E. J.
  102. Agric Biol Chem v.53 Cloning and expression in Esherichia coli of the gene for rat tumor nercrosis factor Shirai, T.;Shimizu, N.;Horiguchi, S.;Ito, H.
  103. Proc. Natl. Acad. Sci. USA v.83 Substance P receptors in primary cultures of cortical astrocytes form the mouse Torrens, Y.;Beaujouan, J. C.;Saffroy, M.;Daguet de Montety, M. C.;Bergstorm, L.;Glowinski, J.
  104. J. Neuroimmunol. v.30 Astrocyte conditioned medium stimulates HIV-1 expression in a chronicaliy infected promonocyte clone Vitkovic, L.;Kalevic, T;de Cunha, A.;Fauci, A. S.