The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Sagunja-Tang on cytotoxicity and lipid peroxidation in rat renal cortical slices

사군자탕(四君子湯) 및 그 구성약물(構成藥物)이 백서(白鼠) 신피질(腎皮質) 절편(切片)의 세포손상(細胞損傷)과 지질과산화(脂質過酸化)에 미치는 영향(影響)

  • Cho, Su-In (Department of Herbology, College of Oriental medicine, Dong-Eui University) ;
  • Kim, Gyung-Chul (Department of Physiology, College of Oriental medicine, Dong-Eui University) ;
  • Lee, Yong-Tae (Department of Physiology, College of Oriental medicine, Dong-Eui University)
  • 조수인 (동의대학교 한의과대학 본초학교실) ;
  • 김경철 (동의대학교 한의과대학 생리학교실) ;
  • 이용태 (동의대학교 한의과대학 생리학교실)
  • Published : 1999.03.30
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Abstract

This study was carried out to investigate whether water extract of Sagunja-Tang and its composing herbs have the inhibitory effects on cytotoxicity and lipid peroxidation induced by oxidant in rat renal cortical slices. Cytotoxicity was estimated by measuring lactate dehydrogenase (LDH) activity and lipid peroxidation was examined by measuring malondialdehyde (MDA). a product of lipid peroxidation. When rat renal cortical slices were treated with tert-butylhydroperoxicle (t-BHP) of 1 mM and water decocted herbs. LDH release from the slices was inhibited in dose dependent manner at low concentrations of herbs. It shows that herbs can reduce cytotoxicity, but overdose of herbs can be toxic to the slices. And MDA measurements show each herb has its own activities of preventing cytotoxicity from oxidants. So further studies should be followed to make clear the mechanisms of anti-oxidative effects of herbs.

Keywords

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