Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
DOI QR코드

DOI QR Code

The Effects of t-butylhydroperoxide (tBHP) and Juglandis Semen on Brain Na+-Pump Activity

호도약침액(胡桃藥鍼液)이 가토(家兎) 뇌조직(腦組織)의 Na+-pump 활성(活性) 장애(障碍)에 미치는 영향(影響)

  • Kim, Dong-Hoon (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Jang, Kyung-Jeon (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Song, Choon-Ho (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV) ;
  • Ahn, Chang-Beohm (Dept. of Acu. & Mox., College of Oriental Medicine, Dong Eui UNIV)
  • 김동훈 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Published : 1999.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to determine whether Juglandis Semen (JAS) extraction exerts protective effect against oxidant-induced inhibition of $Na^+$-pump activity in cerebral cortex. $Na^+$-pump activity was estimated by measuring ouabain-sensitive oxygen consumption. The oxygen consumption significantly inhibited by 1 mM t-butylhydroperoxide (tBHP), which was prevented by addition of 2% JAS extraction. The oxygen consumption was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10 mM, and $Mg^{++}$ concentration from 0.2 to 5 mM. These changes in ion concentrations did not affect the inhibitory effct of tBHP and protective action of JAS on oxygen consumption. tBHP(1mM) produced a significant increase in lipid peroxidation in cerebral cortex, which was prevented by 2% JAS extraction. These result suggest that JAS exerts protective effect against tBHP-induced inhibition of $Na^+$-pump activity in the cerebral cortex, this effect may be due to by an antioxidant action.

Keywords

References

  1. Ann Rev. Biochem. v.36 Biochemical aspects of active transport Albers, R.W. https://doi.org/10.1146/annurev.bi.36.070167.003455
  2. The sodiurm-potassium adenosinetriphosphatase, Metabolic Pathways(3rd ed.) Vl. Metabolic Transport Hokin, L.E.;Dahl, J.L.;Hokin, K.E.(ed.)
  3. Pharmacol. Rev. v.27 The sodium-potassium adenosine triphosphatase, Phamacological, physiological and biochemical aspects Schwartz, A.;Lindenmayer, G.E.;Allen, J.C.
  4. Trends-Neunosci. v.16 A radical hypothesis for neurodegenration Olanow, C. W. https://doi.org/10.1016/0166-2236(93)90070-3
  5. Physiol. Rev. v.59 Hydroperoxide metabolism unmammalian organs Chance, B.;Sies, H.;Boveris, A. https://doi.org/10.1152/physrev.1979.59.3.527
  6. Morphological characterization science v.175 Microsomal lipid peroxidation Arstila, A. U.
  7. Am. J. Physiol. v.254 Depression of membrane-bound Na+-K+-ATPase activity induced by free rqudicals and by ischemia of kidney Koko, K.;Kato, M. Matsuoka, T.; Mustapha, A. https://doi.org/10.1152/ajpcell.1988.254.2.C330
  8. Mol-Cell-Biochem. v.126 Phosphatidyl serire synthosis in rat cerevral cortex:effects of hypoxia, hypocapnia and development Andreoli, V. https://doi.org/10.1007/BF00925687
  9. Biophys. Acta. v.266 The effect of lipoxidation on synpatosomal(Na+K+)-ATPase isolated from the cerebral cortex of squiral monkey Sun, A.Y. https://doi.org/10.1016/0005-2736(72)90093-4
  10. 精校黃帝內經素問 洪元植
  11. 精校黃帝內經靈樞 洪元植
  12. 東醫心系內科學 具本泓;李京燮;裵亨燮;金永錫;李源哲
  13. 本草備要解析 楊東喜
  14. 本草綱目(下卷) 李時珍
  15. 本草求眞 皇宮
  16. 東醫臨床方劑學 尹吉榮
  17. 대한약전의 한약(생약)규격집 지형주;이상인
  18. 大韓鍼灸學會誌 v.13 no.2 胡桃藥鍼液의 抗酸化效果에 對한 硏究 ll oxidant에 依한 細胞損傷을 防止하는 機轉 金永海;金甲成
  19. 胡桃水鍼이 家兎腎臟의 抗酸化酵素活性에 미치는 影響 姜亨定
  20. Anal. Biochem. v.72 A rapid and sensitive method for the quatitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  21. Anal. Biochem. v.86 Determination of malonaldehyde precusor in tissue by thiobarbituric acid test Uchiyama, M.;Mihara, M. https://doi.org/10.1016/0003-2697(78)90342-1
  22. Cochran Statirticol methods(6th ed.) Snedecor, G.H.;W.G.
  23. 原色臨床本草學 辛民敎
  24. 圖說 韓方藥理·藥能의 臨床應用 李載熙
  25. 韓藥臨床應用 李尙仁(外)
  26. Merck Index twelfth edition Susan, Budavari.
  27. 生理學 3版 2장 姜斗熙
  28. Eur. J. Pharmacol. v.55 Effects of monovalent cations on(Na++K+)-ATPase in rat brain slices Akera, T.;Gubitz, R.H.;Brody, T.M.; Tobin, T. https://doi.org/10.1016/0014-2999(79)90196-1
  29. Biochem. Biophy. Acta. v.459 Control of brain slice respiration by (Na++K+)-activated adenosine triphosphatase and the effects of enzyme inhibitors Gubitz, R.H.;Akera, T.;Brody, T.M. https://doi.org/10.1016/0005-2728(77)90027-5
  30. FASEB J. v.4 Role of Oxygen free radicals in carcinogenesis and brainischemia Floyd, R.A. https://doi.org/10.1096/fasebj.4.9.2189775
  31. FASEB J. v.9 Oxidative processes and antioxidative defense mechanisms in the againg brain Reiter, R.J. https://doi.org/10.1096/fasebj.9.7.7737461
  32. N-ENGL-J-Med. v.312 Oxygen-derived free radicals in post-ischemic tissue injury Mccord, J.M. https://doi.org/10.1056/NEJM198501173120305
  33. Acta. Neurol. Scand. v.126 Oxidants and the central nervous system-some fundamental questions. Is oxidant damage relevant to Parkinson's disease, Alzheimer's disease, traumatic injury or stroke? Halliwell, B.
  34. J-Apple-physiol. v.71 Oxygen radical mechanisms of brain injury following ischemia and repefusion Traystman, R.J. https://doi.org/10.1152/jappl.1991.71.4.1185
  35. Free-Radic-Biol-Med. v.14 The relationship between excitotrxicity and oxidative stress in the CNS Brondy, S.C.;Lebel, C.P. https://doi.org/10.1016/0891-5849(93)90144-J
  36. Biochem. Pharmacol. v.49 Antioxidant characterization. methology and mechanism Halliwell, B. https://doi.org/10.1016/0006-2952(95)00088-H
  37. Proc-Soc-Exp-Biol-Med. v.209 Mechanisms of oxidant stress-induced acute tissue injury Jaeschke, H. https://doi.org/10.3181/00379727-209-43885b
  38. Biophys. Acta. v.42 Further investigation on a Ma+++Na+-activated adenosine triphosphatase, possibly related to the active, linked transport of Na+ and K+ across the nerve membrane Skou, J.C. https://doi.org/10.1016/0006-3002(60)90746-0
  39. Biochem. J. v.93 Vectoral aspects of adenosine-triphosphatase activity in erythrocyte membrane Whittam, R.;Ager, M.E. https://doi.org/10.1042/bj0930337