Bacillus subtilis var. globigii 종균접종 메주로 제조한 콩 품종별 간장의 품질 특성

Quality Characteristics of Kanjang Prepared with Meju Cultivated on Different Soybean Cultivars with Bacillus subtilis var. globigii Seed Culture

태광콩, 검정콩1호, 신팔달콩2호, 황금콩, 단백콩, 단엽콩을 공시재료로 하고 Bacillus subtilis var. globigii를 메주제조용 균주로 사용하여 간장을 제조하고 그 성분 특성을 조사하였다. 원료 콩의 조단백질 함량은 단백콩이 47.5%로 가장 높았으며, 유리당 함량은 검정콩1호와 단백콩이 각각 15.87%, 13.33%로 가장 높았다. Bacillus subtilis var. globigii 종균을 접종하여 만든 메주로 제조한 간장의 총질소함량은 단백콩으로 제조한 간장에서 1.18%로 가장 높았으며, 간장의 유리당과 유기산 함량은 콩 품종간에 큰 차이를 보이지 않았으며 succinic acid가 주된 유기산임을 보여주었다. 단백콩 간장의 총 유리아미노산과 glutamic acid 함량도 각각 3365 mg%와 737.44 mg%로 가장 높았다. 원료콩의 단백질 함량이 가장 높은 단백콩이 간장의 총 유리아미노산, glutamic acid 함량 및 총 아미노산에 대한 glutamic acid의 비율이 가장 높아서 간장용 콩 품종으로 가장 적합한 것으로 나타났다.

This study was carried out to select the most suitable soybean cultivars for the quality kanjang preparation. Six cultivars of soybean, Taekwangkong, Kumjungkong #1, Sinpaldalkong #2, Hwangkeumkong, Danbaegkong and Danyeobkong, were used as the raw materials for kanjang preparations. Grain type meju was prepared by the inoculation of Bacillus subtilis var. globigii seed culture on the cooked soybean of respective cultivar and cultivation at $30^{\circ}C$ for 10 days. Two month matured respective kanjang mash, which was prepared by mixing meju and 20% salt brine in the ratio of 1 : 3, was separated and the liquid portions were used as kanjang samples for this work. The highest crude protein content of 47.5% was obtained from Danbaegkong cultivar among six soybean cultivars tested. High sugar soybean cultivars were found to be Kumjungkong l and Danbaegkong and the content 15.87% and 13.33% respectively. The highest total nitrogen(TN) content of 1.18% was shown from Danbaegkong kanjang. Although the major free organic acid in kanjang was found to be succinic acid, no significant differences in free organic acid and sugars content were observed among cultivars. The highest free total amino acids(TA) and glutamic acid(GA) content in kanjang was observed to be 3365 mg% and 734.44 mg% respectively from Danbaegkong kanjang. Danbaegkong cultivar soybean which was shown to contain the highest crude protein was found to be the most suitable one for the preparation of quality kanjang with high TN, GA content and GA/TA ratio.