Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of CMCase Isozymes from Phytopathogenic Erwinia chrysanthemi PY35

무름병균 Erwinia chrysanthemi PY35의 CMCase isozymes 분리

  • Park, Sang-Ryeol (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Cho, Soo-Jeong (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Yun, Han-Dae (Department of Agricultural Chemistry, Gyeongsang National University)
  • Published : 1999.08.31
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Abstract

Soft-rot bacterial pathogen, Erwinia sp., was isolated from chinese cabbage tissue showing soft-rot symptom. This bacterial strain caused soft-rot to chinese cabbage and potato, and identified as Erwinia chrysanthemi PY35(Ech PY35). Ech PY35 have extracellular CMCase, pectinase, pectate lyase, and protease activity, but not hemicellulase activity. The results of the microscopy showed that chinese cabbage tissue and potato tissue were macerated by infection of Ech PY35. In analysis of the CMCases activity in the total protein of Ech PY35, three CMCases were detected as intracellular protein while two CMCases were as extracellular protein by CMC-SDS-PAGE direct stain method.

배추로부터 연부균을 분리하여 조사한 결과 Erwinia chrysanthemi(Ech)로 동정되었으며 분리균 Ech PY35는 CMCase, pectinase, pectate lyase, protease 활성은 있었으나 hemicellulase 활성은 없었다. 배추와 감자의 조직에서 $24{\sim}48$시간 이내에 연부증상을 일으키며, 배추 조직보다 감자 조직에서 연부증상의 발병 진행속도가 느린 편이었다. 전자현미경관찰 결과 병원균의 식물 조직 내의 침투가 확인되었으며 이들이 분비한 세포벽분해효소에 의한 식물조직의 연부현상도 관찰되었다. 분리균의 체내 및 체외효소를 분리하여 CMC-SDS-PAGE에 의한 CMCase 직접활성 염색법을 이용하여 5종류의 CMCase isozyme으로 추정되는 활성밴드를 관찰할 수 있었다. 체내분비효소로 48 kDa, 34 kDa, 31 kDa의 3종류, 체외분비효소는 50 kDa, 29 kDa 2종류로 확인되었다.

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