Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 31 Issue 6
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- Pages.1648-1653
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- 1999
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- 0367-6293(pISSN)
Isolation and Identification of Fibrinolytic Enzyme Producing Strain from Shrimp Jeot-Gal, a Tiny Salted Shrimps, and Medium Optimization for Enzyme Production
새우젓에서 혈전용해효소 생산균주의 분리, 동정 및 효소생산 배지의 최적화
- Jang, Sun-Ae (Department of Food and Nutrition, Chungnam National University) ;
- Kim, Myung-Hee (Environmental Bioresources Lab., Korea Research Institute of Bioscience and Biotechnology) ;
- Lee, Myung-Sun (Department of Food and Nutrition, Chungnam National University) ;
- Lee, Myung-Ja (Department of Food and Nutrition, Chungnam National University) ;
- Jhee, Ok-Hwa (Department of Food and Nutrition, Chungnam National University) ;
- Oh, Tae-Kwang (Environmental Bioresources Lab., Korea Research Institute of Bioscience and Biotechnology) ;
- Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University)
- 장순애 (충남대학교 식품영양학과) ;
- 김명희 (생명공학연구소 환경생물소재연구실) ;
- 이명선 (충남대학교 식품영양학과) ;
- 이명자 (충남대학교 식품영양학과) ;
- 지옥화 (충남대학교 식품영양학과) ;
- 오태광 (생명공학연구소 환경생물소재연구실) ;
- 손천배 (충남대학교 식품영양학과)
- Published : 1999.12.31
Abstract
A strain of potential producer of fibrinolytic enzyme was isolated from shrimp Jeot-Gal, a tiny salted shrimps, and identified as Bacillus sp.. The preliminary experiment showed an enzyme yield of 18 U/mL in medium for screening. The carbon, nitrogen and salts significantly influenced the fibrinolytic enzyme production. An optimized medium containing 2% skim milk, 2% soluble starch and 3% NaCl (pH 7.5) after 72 hrs fermentation time at
새우젓으로부터 혈전용해효소를 생산하는 균주를 분리하여 Bacillus sp.로 동정하였다. Bacillus sp. S19는 screening 배지에서는 18 U/mL의 효소를 생산하였다. 그러나 2% soluble starch, 2% skim milk, 3% NaCl(pH 7.5)의 최적화된 배지로