한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제31권5호
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- Pages.1386-1391
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- 1999
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- 0367-6293(pISSN)
치자 열매에서 항미생물 활성을 갖는 3,4-Dihydroxybenzoic Acid의 분리
Isolation of 3,4-Dihydroxybenzoic Acid, Which Exhibits Antimicrobial Activity, from Fruits of Gardenia jasminoides Ellis
- Yim, Cheol-Keun (Department of Food Science and Technology, Chonnam National University) ;
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Moon, Jae-Hak
(Department of Nutrition, University of Tokushima) ;
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Park, Keun-Hyung
(Department of Food Science and Technology, Chonnam National University)
- 발행 : 1999.10.31
초록
치자(Gardenia jasminoides Ellis) 열매의 methanol추출물은 Gram양성 세균, Gram음성 세균, 효모 등의 미생물에 대하여 항미생물 활성을 보여, solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, ODS column chromatography, HPLC등에 의해 얻어진 활성 물질을 MS, GC-MS,
The methanol extract of Gardenia jasminoides Ellis showed antimicrobial activity against bacteria and yeasts. The extract was successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, octadecylsilane column chromatography. The purified active substance was isolated by high performance liquid chromatography. The isolated compound was 3,4-dihydroxybenzoic acid which was determined by mass spectrometer, gas chromatograph-mass spectrometer,