우슬로부터 20-Hydroxyecdysone의 분리 및 함량분석

Isolatin and Quantitative Determination of 20-Hydroxyecdysone from Achyranthis Radix

  • 발행 : 1999.09.30

초록

Separation and quantitative determination of 20-hydroxyecdysone from Achyranthis Radix has been conducted by using HPLC method. 20-hydroxyecdysone in a methanol extract from the raw drug was separated on a reverse phase column using a $CH_3CN-H_2O\;(18:82)$ solvent system and the average content is $0.0931{\pm}0.0048%$. For the preparation of authentic standard, we isolated 20-hydroxyecdysone from the roots of Achyranthes fauriei by $SiO_2$ and RP-18 column chromatography.

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