생약학회지 (Korean Journal of Pharmacognosy)

  • 제30권3호
  • /
  • Pages.255-260
  • /
  • 1999
  • /
  • 0253-3073(pISSN)
  • /
  • 2288-9299(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

이공산(異功散)의 세포보호 및 항산화 작용

Cytoprotective and Antioxidative Effects of Crude Drug Preparation (E-kong-san)

  • 이경태 (경희대학교 동서의학 연구소) ;
  • 최정혜 (경희대학교 약학대학) ;
  • 노영수 (경희대학교 약학대학) ;
  • 안규석 (경희대학교 한의과대학, 동서의학 연구소) ;
  • 장성구 (경희대학교 의과대학, 동서의학 연구소) ;
  • 오수명 (경희대학교 의과대학, 동서의학 연구소) ;
  • 정지창 (경희대학교 의과대학, 동서의학 연구소)
  • Lee, Kyung-Tae (East-West Medical Research Institute, Kyung Hee University) ;
  • Choi, Jung-Hye (College of Pharmacy, Kyung Hee University) ;
  • Rho, Young-Soo (College of Pharmacy, Kyung Hee University) ;
  • Ahn, Kyoo-Seok (Oriental Medicine, East-West Medical Research Institute, Kyung Hee University) ;
  • Chang, Sung-Goo (College of Medicine, East-West Medical Research Institute, Kyung Hee University) ;
  • Oh, Soo-Myung (College of Medicine, East-West Medical Research Institute, Kyung Hee University) ;
  • Jung, Jee-Chang (College of Medicine, East-West Medical Research Institute, Kyung Hee University)
  • 발행 : 1999.09.30
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초록

In the previous report, E-kong-san, which is usually used for recovering health in traditional medicine, has been shown to decrease cisplatin induced nephrotoxicity in vivo and in vitro. The significant reduction of E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of triggering antioxidation. In monkey kidney Vero cells, E-kong-san at $5{\sim}10\;mg/ml$ was able to attenuate 2mM cisplatin-stimulated cell death by 46.8% and 31.8%, respectively. E-kong-san showed strong free radical scavengering activities on 1,1-diphenyl-2-picrylhydrazil (DPPH) radical and xanthine/xanthine oxidase (XOD) generated superoxide anion radical $(O_2^{-.})$. We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. Moreover, E-kong-san exhibited significant inhibition on both ascorbic $acid/Fe^{2+}$ and $ADP/NADPH/Fe^{3+}$ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that E-kong-san protects the cisplatin induced cytotoxicity by its antioxidative mechanism.

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