Natural Product Sciences
- Volume 5 Issue 3
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- Pages.113-120
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- 1999
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- 1226-3907(pISSN)
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- 2288-9027(eISSN)
Effects of Natural Products on the Inhibition of Lipopolysaccharide-Inducible Nitric Oxide Synthase Activity in RAW264.7 Cell Culture System
- Park, Bong-Joo (Natural Products Research Institute, Seoul National University) ;
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Cho, Myung-Haing
(College of Veterinary Medicine, Seoul National University) ;
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Kim, Kyeong-Ho
(College of Pharmacy, Kangwon National University) ;
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Lee, Sang-Kook
(College of pharmacy, Ewha Womans University) ;
- Lee, Chong-Soon (Department of Biochemistry, College of National Sciences, Yeungnam University) ;
- An, Gil-Hwan (Department of Applied Biology, Sunmoon University) ;
- Mar, Woong-Chon (Natural Products Research Institute, Seoul National University)
- Published : 1999.09.01
Abstract
Nitric oxide (NO) is a free radical synthesized from L-arginine by nitric oxide synthase (NOS). It is believed that NO is an important mediator in numerous physiological and inflammatory responses. Particularly, a large amount of NO released from the inducible nitric oxide synthase (iNOS) is mostly associated with inflammatory processes. Overproduction of NO in these processes including sepsis and autoimmune diseases can have deleterious consequences and pathophysiologic relevance. Therefore, for the discovery of new inhibitory agents against iNOS activity, we have evaluated about 100 kinds of natural products after partition into three layers (n-hexane, ethyl acetate and aqueous) from 100% methanol extracts to study inhibitory effects on iNOS activity induced by lipopolysaccharide (LPS) in RAW264.7 cells culture system. As a positive control, curcumin, which is known as an anti-tumor promoter, anti-inflammatory agent as an iNOS inhibitor, was used and showed the dose-dependent inhibitory effect