Major Chemical Constituents of Supercritical Carbon Dioxide Extract of Pandanus odorus Leaves

Supercritical carbon dioxide was used as a solvent in the extraction of freeze-dried Pandanus odorus leaves. Analysis of the extract with GC-MS showed that Pandanus odorus leaves contain nutritional constituents such as ${\alpha}-tocopherol$ (Vitamin E) and squalene. The contents of ${\alpha}-tocopherol$ and squalene extracted from freeze-dried ground Pandanus odorus leaves at pressures ranging from 80 to $200\;kg\;cm^{-2}$ and temperatures between 40 to $80^{\circ}C$ were $134{\sim}300\;ppm$ and $750{\sim}1,200\;ppm$ respectively. The highest yield was obtained at $200\;kg\;cm^{-2}$ and $40^{\circ}C$. Other major components identified were hexadecanoic acid, 9,12,15-octadecatrien-1-ol, campesterol, stigmasterol and ${\beta}-sitosterol$.