The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Chemical Composition and Biological Activities of Immunostimulants Purified from Alkali Extract of Poria cocos Sclerotium

복령 균핵의 알칼리추출물에서 정제한 면역활성 증강물질의 작용과 화학구성

  • Rhee, Sang-Dal (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA) ;
  • Cho, Soo-Muk (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Jeong-Sik (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA) ;
  • Han, Sang-Bae (Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon, Young-Jin (Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hwan-Mook (Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Gwang-Po (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA)
  • 이상달 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 조수묵 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 박정식 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 한상배 (생명공학연구소 유전자원센터) ;
  • 전영진 (생명공학연구소 유전자원센터) ;
  • 김환묵 (생명공학연구소 유전자원센터) ;
  • 김광포 (농촌진흥청 농업과학기술원 응용미생물과)
  • Published : 1999.08.30
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Abstract

An one percent sodium carbonate extract prepared from sclerotia of Poria cocos activated the proliferation of the T lymphocytes as measured by mixed lymphocyte responses(MLR). The active fraction, PCSC22, was isolated from an one percent sodium carbonate extract by a combination of fractionation procedures, including ethanol precipitation and chromatographies on column of DEAE-cellulose and Sephadex G50. Carbohydrate and peptide contained in PCSC22 were 78 : 22% in ratio. On employing gel filtration high performance liquid chromatography, PCSC22 exhitited a homogeneous peak with an average molecular weight of 8 kDa. The sugar moiety of PCSC22 was composed with mannose (92%), galactose (6.2%) and arabinose (1.3%), which might be indicated as heteromannan. Fifteen amino acids were found in peptide moiety of the polysaccharide and aspartic acid, serine, and valine were major components. PCSC22 activated the primary proliferation of T lymphocytes measured by mixed lymphocyte responses, the antibody production of the B lymphocytes and the secretion of nitric oxide from macrophage cell line, RAW264.7.

복령 균핵을 여러단계로 추출하여 얻은 다당류들의 면역 증강활성을 생쥐 비장세포에서 혼합임파구 반응으로 측정하였다. 이들 중 탄산나트륨 추출물의 활성이 가장 높았고 이를 DEAE-셀롤로오스와 Sephadex G-50 크로마토그래피로 면역활성증강물질을 정제하여 PCSC22를 얻었다. PCSC22는 탄수화물과 단백질이 약 78:22의 비율로 이루어진 단백다당체이었으며 GP-HPLC로 측정된 평균분자량은 약 8 kDa이었다. PCSC22 분획은 단당들의 구성성분들 중 mannose(92%)가 가장 많고 galactose(6.2%)와 약간의 arabinose(1.3%)도 함유하고 있는 heteromannan 이었다. 단백질 부분은 총 15종의 아미노산으로 구성되어 있었고 그 중 aspartic acid, serine, valine 등의 합이 약 47%로 주요 성분이었다. PCSC22는 B 임파구의 항체형성능력과 macrophage의 일산화질소 분비능력을 함께 증강시켰다.

Keywords

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