한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
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Aspergillus ficuum의 Phytase의 정제와 Anti-phytase 항체생산

Purification of Phytase from Aspergillus ficuum and Production of Anti-phytase Antibody

  • 김근 (수원대학교 유전공학과)
  • Kim, Keun (Department of Genetic Engineering, The University of Suwon)
  • 발행 : 1999.08.30
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초록

Aspergillus ficuum이 발효조의 배양액에서 phytase를 가장 많이 분비 생산하는 시간은 5일째였다. 이 phytase를 ultrafiltration, cation exchange column, 그리고 anion exchange column으로 최종 정제하여 SDS-PAGE로 분석하여 본 결과 단일 band로 약90,000의 분자량을 가진 것으로 나타났다. 정제된 phytase를 사용하여 phytase에 대한 항체를 생산하였고 titer는 1/25,000로 결정하였다.

Phytase(myo-inositol-hexakis phosphate 3-phosphohydrolase, E C 3.1.3.8) sequentially hydrolyzes phytate to myo-inositol and inorganic phosphate. Phytase of Aspergillus ficuum was purified to homogeneity using ultrafiltration, cation exchange column and anion exchange column. It's molecular weight is estimated as around 90,000 by SDS-PAGE. Antibody against the phytase was produced by immunizing mice with the purified phytase. The titer of the antibody was determined to be 1/25,000.

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참고문헌

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