The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification and Characterization of Osmotolerant Yeast Isolated from Soy Paste

된장에서 분리된 내염성 효모의 동정 및 특성조사

  • 변명옥 (농업과학기술원 분자유전과) ;
  • 이승범 (농업과학기술원 분자유전과) ;
  • 구본성 (농업과학기술원 분자유전과) ;
  • 송재경 (농업과학기술원 분자유전과) ;
  • 류진창 (농업과학기술원 분자유전과) ;
  • 이두형 (서울시립대학교 환경원예학과)
  • Published : 1999.06.30
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Abstract

Osmotolerant yeast isolated from soy paste could grow on media with 2 M NaCl. This strain was identified as Zygosaccharomyces rouxii by biological characteristics, RFLP of ribosomal DNA and mating with compatible haploid strain. Growing rate of the Z. rouxii YDJ was slower than Saccharomyces cerevisiae. Z. rouxii YDJ accumulated trehalose, which is known as one of the osmolytic protectants, in cells cultured on media with salt. Enzyme activity of trehalose phosphate synthase related to trehalose biosynthesis of the YDJ was lower than those of S. cerevisiae. Trehalase activity related trehalose degradation was also lower in Z. rouxii YDJ than S. cerevisiae. However, as Z. rouxii accumulated trehalose by salt treatment, salt tolerancy of Z. rouxii was assumed to be related to trehalose in additon to glycerol.

염농도가 높은 전통식품인 된장에서 호염성 효모를 분리하였다. 2M NaCl에서도 생육할 수 있는 이 균은 Zygosaccharomyces rouxii로 등정되었으며 생리적 특성, rDNA를 probe로 이용한 RFLP 및 화합성 단핵 동종 효모 균주와 교배 결과에 의하여 이루어졌다. Z. rouxii YDJ 균은 생장속도가 S. cerevisiae 보다 느려 대수기를 완료하는데 $2{\sim}3$일 소요되었다. S. cerevisiae에서는 배지에 염농도가 높아질수록 glycerol보다 trehalose 함량이 크게 증가하였으나 Z. rouxii는 세포 내에 glucosamine, glycerol, trehalose함량이 증가하였다. 따라서 Z. rouxii의 호염성은 glycerol과 같은 polyol이외에도 trehalose과 glucosamine의 효과가 크게 나타났다. Trehalose 생합성 효소인 trehalose phosphate synthase는 S. cerevisiae보다 Z. rouxii에서 낮았으며 trehalose 분해효소인 trehalase도 S. cerevisiae 보다 Z. rouxii에서 낮았다. 그러나 Z. rouxii는 NaCl 처리시 trehalose가 증가하므로 내염성은 glycerol 이외에 trehalose에 의해서도 영향을 받는 것으로 생각된다.

Keywords

References

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