한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

Ribosomal DNA의 Internal Transcribed Spacer(ITS) 부위의 염기서열분석에 의한 Phellinus속의 계통분석에 관한 연구

Phylogenetic Analysis of the Genus Phellinus by Comparing the Sequences of Internal Transcribed Spacers and 5.8S Ribosomal DNA

  • 정지원 (부산대학교 자연과학대학 미생물학과) ;
  • 김기영 (부산대학교 자연과학대학 미생물학과) ;
  • 하명규 (기초과학지원연구소 부산분소) ;
  • 이태호 (부산대학교 자연과학대학 미생물학과) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Chung, Ji-Won (Department of Microbiology, College of Natural Science, Pusan National University) ;
  • Kim, Gi-Young (Department of Microbiology, College of Natural Science, Pusan National University) ;
  • Ha, Myung-Gui (Korea Basic Science Institute) ;
  • Lee, Tae-Ho (Department of Microbiology, College of Natural Science, Pusan National University) ;
  • Lee, Jae-Dong (Department of Microbiology, College of Natural Science, Pusan National University)
  • 발행 : 1999.04.30
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초록

본 실험은 진흙버섯류 7종 15균주에 대한 5.85 rDNA와 ITS 부위의 염기서열을 비교 분석함으로서 종간 및 종내의 유연관계를 조사하였다. 5.8S rDNA와 ITS 부위를 증폭하고자 18S rDNA의 3'말단 부위와 28S rDNA의 5'말단 부위에 두 개의 primer를 이용하여 PCR증폭을 행하였다. 5.8S rDNA와 ITS 부위를 증폭하여 염기서열을 비교 분석한 결과 본 실험에 공시된 Phellinus속의 제균종은 크게 4개의 cluster를 형성하였다. 첫 번째 cluster는 Phellinus hartigii IMSNU 32041, Phellinus robustus IMSNU 32068로 이루어졌고, 두 번째 cluster는 Phellinus linteus KCTC 6190, IMSNU 31014, DGUM 25003, DGUM 25004, Phellinus sp. DGUM 25007, Namsan No. 1과 Phellinus weirianus IMSNU 32021, 세 번째 cluster는 Phellinus laevigatus KCTC 6229, KCTC 6230과 Phellinus igniarius KCTC 6227, KCTC 6228로 이루어졌으며, Phellinus chrysoloma KCTC 6225와 KCTC 6226이 마지막 cluster를 형성하였다. 결과적으로 ITS 염기서열의 결과만으로 볼 때 Phellinus linteus와 Phellinus weirianus는 명확하게 종 단위의 개념을 정립할 수 없었다. 따라서 정확한 분류를 위해 생리학적, 분자생물학적 인 분류방법이 첨가되어야 하며, type strain에 대한 ITS 염기서얼도 결정되어야 한다. Phellinus속의 균들에서는 ITS2부위에 비해 ITS1부위의 변이율이 높았다. ITS 염기서열은 종 구분에 유용한 도구이며, 다른 균종들과 비교해 보았을 때 Phellinus linteus와 Phellinus weirianus에서만 ITS1 부위에서 특이적인 염기서열을 가지고 있었다.

This study was carried out to identify the phylogenetic relationship among Phellinus species by comparing the DNA sequences of the 5.8S ribosomal DNA (rDNA) and the internal transcribed spacers (ITSs), ITS1 and ITS2 regions. Two primers from the 3' end of 18S rDNA and the 5' end of 28S rDNA sequences were chosen to amplify the specific ITS regions of Phellinus spp. Phellinus strains used in the study were divided into four clusters by the phylogenetic tree based on the amplified regions of ITS and 5.8S rDNA sequences. The first cluster consist of Phellinus hartigii IMSNU 32041 and Phellinus robustus IMSNU 32068, and the second cluster consists of Phellinus linteus strains and Phellinus weirianus IMSNU 32021. Phellinus laevigatus KCTC 6229, KCTC 6230 and Phellinus igniarius KCTC 6227, KCTC 6228 belong to the third cluster. Finally, Phellinus chrysoloma KCTC 6225 and Phellinus chrysoloma KCTC 6226 are the fourth cluster. In the second cluster the differentiation between Phellinus linteus strains and Phellinus weirianus species were not possible by the comparison of the ITS sequences. These results revealed that Phellinus linteus and Phellinus weirianus cannot be established the concept of species level only by the ITS sequences. Therefore, both physiological and molecular biological methods as well as the sequences of type strains are necessary to classify the strains of these two species accurately. The comparison of the ITS sequences of four Phellinus species indicated that the sequences of the ITS1 generally are more divergent than those of the ITS2. Although the ITS sequences are varied in some species, the conserved regions in both ITS1 and ITS2 are useful tool to differentiate the species. Phellinus linteus and related species have their specific sequences in the ITS1 compared to the other species.

키워드

참고문헌

  1. 한국균학회지 v.24 no.2 고등균류 균사체의 면역조절 가능성에 관한 연구 배만종;박무희;이재성
  2. 목질진흙버섯(Phellinus linteus)의 균사체 배양을 위한 최적 조건 및 균사의 효소활성 이창윤
  3. 한국미생물학회지 v.32 미토콘드리아 DNA의 제한효소 분석법에 의한 진흙버섯류의 계통분류 정원진;정학성
  4. 미생물과 산업 v.15 목재부후균류(담자균 민주름버섯목)의 분류학적 고찰 정학성
  5. Mycologia v.88 no.5 Internal transcribed spacer sequences from 38 recognized species of Suillus sensu lato: Phylogenetic and taxonomic implications Annette, K.;Li, Y.;Szaro, T.;Bruns, T.D.
  6. Kor. J. Mycol. v.24 Phylogenetic relationships among Pleurotus species species inferred from sequence data of PCR amplified ITS II region in ribosomal DNA Bae, S.C.;Seong, K.Y.;Lee, S.W.;Go, S.J.;Eun, M.Y.;Rhee, I.K.
  7. Appl. Env. Microbiol. v.62 DNA polymorphism in Morels: Complete sequences of the Internal Transcribed Spacer of genes coding for rRNA in Morchella esculenta(yellow morel) and Morchella conica(black morel) Daniel, W.;Munch, J.C.;Botton, B.;Buscot, F.
  8. Persoonia v.3 A conspectus of the families of Aphyllophorales Donk, M.A.
  9. Mycologia v.72 Wood-rotting fungi of North America Gilbertson, R.L.
  10. North America polypores. Vol. I Gilbertson, R.L.;Ryvarden, L.
  11. J. Gen. Microbiol. v.137 Phylogenetic analysis of five medically important Candida species as deduced on the basis of small ribosomal subunit RNA sequences Hendriks, L.;Goris, A.;Peer, Y.V.;Neers, J.;Vancanneyt, M.;Kersters, K.;Hennebert, G.L.;Wachter, R.
  12. Nucl. Acids Res. v.21 Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride Heng, Z.;Qu, F.;Zhu, L.H.
  13. Biosystem v.19 Evolutionary change in 5S rRNA secondary structure and a phylogenetic tree of 352 5S rRNA species Hori, H.;Osawa
  14. Gann. v.59 Antitumor action of some basidiomycetes, especially Phellinus linteus Ikekawa, J.;Nakanishi, M.;Uehara, N.;Chihara, G.;Fukuoka, F.
  15. J. Immunol. Immunopharmacol. v.11 Immunomodulatory substances of fungal origin Jong, S.C.;Birmingham, J.M.;Pai, S.H.
  16. J. Microbiol. v.35 no.2 Phylogenetic study of Trichaptum inferred from nuclear ribosomal DNA sequences Ko, K.S.;Hong, S.G.;Jung, H.S.
  17. Phellinus (Hymenochaetaceae): A survey of the world taxa Larsen, M.J.;Cobb-Poulle, L.A.
  18. Phellinus(Hymenochaetaceae) Larsen, M.J.;Cobb-Poulle, L.A.
  19. J. Microbiol. Biotechnol. v.6 Immunostimultating activity and characterization of polysaccharides from mycelium of Phellinus linyeous Lee, J.H.;Cho, S.M.;Song, K.S.;Han, S.B.;Kim, H.M.;Hong, N.D.;Yoo, I.D.
  20. Coloured Korean Mushrooms. Vol. I Lee, J.Y.
  21. Tree disease concepts Manion, P.D.
  22. Mycologia v.87 Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences Moncalvo, J.M.;Wang, H.H.;Hseu, R.S.
  23. Plant Pathology v.44 Sequences of the internal transcribed spacers of the ribosomal RNA genes and relationships between isolates of Verticillium alboatrum and V. dahliae Morton, A.;Carder, J.H.;Barbara, D.G.
  24. Mol. Biol. Evol. v.10 Phylogenetic relationships among Taphrina, Saitoella, and other highter fungi Nishida, J.;Sugiyama, J.
  25. Curr Genet v.22 Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum O'Donnell, K.
  26. J. Gen. Appl. Microbiol. v.41 Sequences of ribosomal genes and internal transcribed spacers move three plant parasitic fungi, Eremonium ashbyi, Ashbya gossypii and Nematospora coryli, toward Saccharomyces cerevisiae Robert, M.;Prillinger, H.;Ibl, M.;Himmler, G.
  27. Coloured illustrations of fungi of Japan. Vol. II Imazeki, Rokuya;Hongo, Tsuguo
  28. J. Gen. Appl. Microbiol. v.38 Discrimination of mushrooms in genus Pleurotus by DNA restriction fragment length polymorphisms Sagawa, I.;Tanaka, M.;Nagata, Y.
  29. Gann. v.59 Antitumor studies on some extracts of basidiomycetes Shibata, S.;Nishikawa, Y.;Mei, C.F.;Fukuoka, F.;Nakanishim, M.
  30. Mycologia v.85 Higher taxa of Basidiomycetes:An 18S rRNA gene perspective Swann, E.;Taylor, J.W.
  31. Nucl. Acids Res. v.11 Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and matrix choice Thompson, J.D.;Higgins, D.G.;Gibson, T.J.
  32. Icons of Medical Fungi from China Ying, J.;Mao, X.;Ma, Q.;Zong, Y.;Wen, H.