The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Diversity of Korean Isolates of Pseudomonas tolaasii and WLRO (White Line Reacting Organism) using BOX-, REP-, and ERIC-PCR

BOX-, REP-, ERIC-PCR을 이용한 국내 수집 Pseudomonas tolaasii와 WLRO(White line reacting organism) 균주들의 유전적 다양성

  • Chee, Hee-Youn (Department of Plant Pathology, National Institute of Agricultural Science and Technology, RDA) ;
  • Oh, Se-Jong (Department of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA) ;
  • Lincoln, S.P. (Horticulture Research International)
  • 지희윤 (농업과학기술원 작물보호부 병리과) ;
  • 오세종 (농업과학기술원 생물자원부 응용미생물과) ;
  • Published : 1999.04.30
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Abstract

Genetic diversity of Korean isolates of Pseudomonas tolaasii and WLRO (White line reacting organism) was assessed using BOX-, REP-, and ERIC-PCR analysis. P. tolaasii showed nearly identical band patterns among isolates, whereas considerable DNA polymorphism was found among isolates of WLRO. On the basis of dendogram, WLRO is characterized as a complex group with high degree of genetic differentiation. Genetic relatedness based on repetitive DNA regions was low between P. tolaasii and WLRO isolates.

Pseudomonas tolaasii와 WLRO(White line reacting organism)의 국내 수집 균주들에 대하여 REP, ERIC, BOX-PCR분석을 이용하여 유전적 다양성을 측정하였다. p. tolaasii 균주들은 매우 유사하였으나 WLRO 균주들을 상호간의 큰 차이를 보였다. 유전적 근연관계 분석 결과 WLRO 균주들은 유전적으로 변이가 높은 복합 집단 양상을 나타내었다. p. tolaasii 균주들과 WLRO 균주들 사이의 repetitive DNA 부위의 유전적 근연 관계는 적은 것으로 나타났다.

Keywords

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