Production of Fruiting Body Using Cultures of Entomopathogenic Fungal Species

분리된 동충하초 균주를 이용한 자실체 생산

  • Sung, Jae-Mo (Department of Environmental Biology, Mycological Lab., Kangwon National University) ;
  • Choi, Young-Sang (Department of Environmental Biology, Mycological Lab., Kangwon National University) ;
  • Lee, Hyun-Kyung (Department of Environmental Biology, Mycological Lab., Kangwon National University) ;
  • Kim, Sang-Hee (Department of Environmental Biology, Mycological Lab., Kangwon National University) ;
  • Kim, Yong-Ook (Department of Environmental Biology, Mycological Lab., Kangwon National University) ;
  • Sung, Gi-Ho (Department of Environmental Biology, Mycological Lab., Kangwon National University)
  • 성재모 (강원대학교 자원생물환경학부 균학실험실) ;
  • 최영상 (강원대학교 자원생물환경학부 균학실험실) ;
  • 이현경 (강원대학교 자원생물환경학부 균학실험실) ;
  • 김상희 (강원대학교 자원생물환경학부 균학실험실) ;
  • 김용욱 (강원대학교 자원생물환경학부 균학실험실) ;
  • 성기호 (강원대학교 자원생물환경학부 균학실험실)
  • Published : 1999.02.28

Abstract

One hundred and six Cordyceps cultures including five cultures of Paecilomyces tenuipes were used for production of artificial fruiting body. In the test of artificial fruiting body formation, no fruiting bodies were induced on media containing PDA and ground silkworm pupae with liquid nitrogen. The best fruiting body formation was showed on media which mixed at the ratio of 1 unsticky rice to 3.5 water. But fruiting bodies formed on media mixed at the ratio 1 unpolished rice to 2.5 water. Optimal temperature in inducing artificial fruiting body was at $20^{\circ}C$. Twenty seven isolates were selected as good cultures for production of artificial fruiting body. Maturation of fruiting bodies incubated on rice grain media was completed for about 50 to 65 days.

배양실내에서 인공적으로 자실체의 형성을 유도한 결과 PDA와 번데기분말을 혼합한 배지상에서는 자실체의 형성을 유도할 수 없었다. 쌀을 이용한 곡물 배지에 수분함량을 달리하고 번데기를 첨가하여 시험을 실시한 결과 알락미에서는 곡물:수분함량이 1:3.5의 비율로 한배양기에서 가장 왕성한 자실체 생산을 보여 주었으며 현미의 경우는 곡물: 수분함량이 1:2.5로 조성된 배양기와 번데기를 첨가한 배양기에서 우수한 자실체 형성을 보여주었다. 자실체 형성적정 온도로는 $20^{\circ}C$가 적합한 것으로 밝혀졌다. 현미와 번데기를 첨가하며 조제한 배양기내에서 자실체형성을 유도한 결과 자실체 형성 우수균주로서 최종 C. militaris 25균주, C. scarabaeicola 2균주 등 27개 균주를 선발할 수 있었다. 현미와 누에번데기를 넣어 만든 배양기내에서의 자실체 형성은 약 50일내지 65일 사이에 완전한 형태의 자실체 형성을 볼 수 있었다.

Keywords

References

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