The Korean Journal of Physiology and Pharmacology
- Volume 3 Issue 6
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- Pages.565-570
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- 1999
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Glycochenodeoxycholic Acid Induces Cell Death in Primary Cultured Rat Hepatocyte: Apoptosis and Necrosis
- Chu, Sang-Hui (Department of Pharmacology, College of Medicine, Ewha Womans University) ;
- Park, Wol-Mi (Department of Pharmacology, College of Medicine, Ewha Womans University) ;
- Lee, Kyung-Eun (Department of Pharmacology, College of Medicine, Ewha Womans University) ;
- Pae, Young-Sook (Department of Pharmacology, College of Medicine, Ewha Womans University)
- Published : 1999.12.21
Abstract
Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver injury. Due to the detergent-like effect of the hydrophobic bile acids, hepatocellular injury has been attributed to direct membrane damage. However histological findings of cholestatic liver diseases suggest apoptosis can be a mechanism of cell death during cholestatic liver diseases instead of necrosis. To determine the pattern of hepatocellular toxicity induced by bile acid, we incubated primary cultured rat hepatocytes with a hydrophobic bile acid, Glycochenodeoxycholate (GCDC), up to 5 hours. After 5 hours incubation with