BMB Reports
- 제31권4호
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- Pages.370-376
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- 1998
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- 1976-670X(eISSN)
Establishment of a Binding Assay System for Screening of the Inhibitors of $p56^{lck}$ SH2 Domain
- Kim, Jyn-Ho (Signal Transduction Lab., Mogam Biotechnology Research Institute) ;
- Hur, Eun-Mi (Signal Transduction Lab., Mogam Biotechnology Research Institute) ;
- Yun, Yung-Dae (Signal Transduction Lab., Mogam Biotechnology Research Institute)
- 투고 : 1998.03.28
- 발행 : 1998.07.31
초록
Src-Homology 2 (SH2) domains have a capacity to bind phosphotyrosine-containing sequence context and play essential roles in various cellular signaling pathways. Due to the specific nature of the binding between SH2 domains and their counterpart proteins, inhibitors of SID domain binding have drawn extensive attention as a potential candidate for therapeutic agents. Here, we describe the binding assay system to screen for the ligands or blockers of the SH2 domains with an emphasis on the