BMB Reports
- 제31권4호
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- Pages.333-338
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- 1998
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- 1976-670X(eISSN)
Ceramide-Mediated Cell Death Was Accompanied with Changes of c-Myc and Rb Protein
- Moon, Soon-Ok (Division of Life Science, The University of Suwon) ;
- Lee, Jin-Woo (Division of Life Science, The University of Suwon)
- 투고 : 1998.01.26
- 발행 : 1998.07.31
초록
The sphingomyelin cycle and ceramide generation have been recognized as potential growth suppression signals in mammalian cells. Ceramide has been shown to induce differentiation, cell growth arrest, senescence, and apoptosis. Although the intracelluar target for the action of ceramide remains unknown, recent studies have demonstrated the role of cytosolic ceramideactivated protein phosphatase(CAPP). In this study, the cytotoxic effect of C2-ceramide, a synthetic cellpermeable ceramide analog, on HEp-2 cells and the mechanism by which ceramide induces cell death were investigated. The addition of exogenous C2-ceramide resulted in a concentration dependent cell death. Okadaic acid, a potent inhibitor of CAPP, enhanced ceramide-mediated cell death, which suggests that CAPP is not involved in this process. To understand the mechanism of action of ceramide, we studied the relationship between ceramide and c-Myc and pRb which are defined components of cell growth regulation. Western blot analyses revealed that C2-ceramide (10