대한방사선기술학회지:방사선기술과학 (Journal of radiological science and technology)
- 제21권1호
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- Pages.65-68
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- 1998
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- 2288-3509(pISSN)
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- 2384-1168(eISSN)
Sephadex G-15 또는 G-50 chromatography를 이용한 방사성 동위원소로 표지된 RNA의 정제
Purification of Radiolabeled RNA Using Sephadex G-15 or G-50 Chromatography
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Yoo, Beong-Gyu
(Dept. of Radiotechnology, Wonkwang Health Science College) ;
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Lee, Jong-Seok
(Dept. of Radiotechnology, Wonkwang Health Science College)
- 발행 : 1998.06.30
초록
We attempted to purify radiolabeled RNA using Sephadex G-15 and G-50 chromatography instead of commercial RNA purification kit. In the Sephadex G-15 chromatography the major portion of RNA was eluted in the fractions ranging from 3rd to 5th whereas broad elution profile of RNA was obtained from the Sephadex G-50 chromatography. The elution profile and purity of RNA obtained from Sephadex G-15 chromatography was very similar to that by commercial RNA purification kit. Furthermore, operating time required for purification of RNA by Sephadex G-15 was rather smaller than that by commercial kit. Overall results suggest that the purification of radiolabeled RNA using Sephadex G-15 is more money and time saying than using commercial RNA purification kit.
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