The Journal of Dong Guk Oriental Medicine (동국한의학연구소논문집)
- Volume 7 Issue 1
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- Pages.87-98
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- 1998
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- 1226-556X(pISSN)
Antioxidant action of Bombycis corpus extraction in renal tissues
신장조직(腎臟組織)에서 백강잠 추출물(抽出物)의 항산화(抗酸化) 작용(作用)에 관(關)한 연구(硏究)
- Lee, Moo-Hyung (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
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Yoon, Cheol-Ho
(Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
- Jeong, Ji-Cheon (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
- Published : 1998.08.31
Abstract
This study was undertaken to determine whether Bombycis Corpus extract (Bom) has antioxidant action. Kidney tissues were exposed to t-butylhydroperoxide (t-BHP) to induce oxidative stress. Lipid peroxidation was estimated by measuring malondialdehyde, a product of lipid peroxidation, and cell injury was estimated by measuring lactate dehydrogenase (LDH) release in rabbit renal cortical slices. t-BHP increased lipid peroxidation and LDH release in a dose-dependent manner over the concentration range of 0.1-1 mM. Such effects of t-BHP on lipid peroxidase and LDH release were prevented by 0.5% Bom. When tissues were treated with t-BHP in the presence of various concentrations of Bom, lipid peroxidation and LDH release were dose-dependently inhibited by Bom. Bom at 1 and 2% concentrations inhibited lipid peroxidation and LDH release in normal tissues. Bom at 2% concentration increased glutathione peroxidase activity in tissues treated or untreated with 1.0 mM t-BHP. However, catalase activity was not altered by addition of Bom. Bom inhibited generation of reactive oxygen species. These results indicate that Bom inhibits lipid peroxidation and cell injury in tissues treated with or without oxidant and this effect is, at least in part, attributed to increased activity of glutathione peroxidase and a direct sacvenging action.
백강잠 추출물이 신장조직에서 반응성 산소기들에 의한 지질의 과산화와 세포 손상을 방지하는지를 관찰하기 위하여 정상조직과 t-BHP를 처리한 조직에서 백강잠 추출물의 효과를 조사하였다. 지질의 과산화와 LDH 유출은 t-BHP의 농도에 비례하여 증가되었으나, 백강잠 추출물의 첨가로 유의하게 억제되었다. 그리고, t-BHP에 의한 지질의 과산화 및 LDH 유출은 백강잠 추출물의 농도에 비례하여 억제되었다. 또한, 백강잠 추출물은 oxidant를 처리하지 않은 정상조직에서도 지질의 과산화 및 LDH 유출을 유의성 있게 감소시켰다. 그리고, catalase 활성에는 영향이 없는 반면, 정상 조직과 t-BHP를 처리한 조직에서는 glutathione peroxidase 활성을 유의성 있게 증가시켰다. 한편, 반응성 산소기의 발생은 백강잠 추출물의 농도에 비례하여 억제되었다. 이러한 결과로 볼 때, 백강잠 추출물은 신장 조직내 항산화 효소의 활성을 증가시킴과 동시에 직접 반응성 산소기의 발생을 억제하므로 신장 조직에서 지질의 과산화와 세포손상을 방지하는 효과가 있을 것으로 여겨진다.