Anti-diabetic effect and effect on glucose-phosphorylase activities of the leaf of Eriobotryae folium on diabetes mellitus mice induced by interleukin-1β

비파엽(枇杷葉)의 항당뇨병약리활성(抗糖尿病藥理活性)과 IL-1β유발당뇨병(誘發糖尿病) 마우스의 췌장(膵臟) 인산화효소(燐酸化酵素)에 미치는 효과(效果)에 관(關)한 연구(硏究)

Studies were conducted on anti-dibetic effect of the water extract from leaves of Eriobotryae folium, which had been used in Korea as a remedy for dibetes. The extract was found to inhibit the increase in the plasma level of sugar bu the not the decrease in the plasma level of insulin in alloxan-induced dibetic rats. Also, we investigated the in vivo effect of an aqueous extract (referred to as EF) from Eriobotryae folium on glucokinase and hexokinase activities of diabetes mellitus induced by interleukin-$1{\beta}$ ($IL-1{\beta}$). After 1 week of $IL-1{\beta}$ injection, the levels of serum glucose concentration and insulin secretion were dramatically increased. However, the insulin secretion was decreased with administration of EF. The level of glucose concentration was decreased by EF administration. Furthermore, it was observed that EF was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase, which are key enzymes of glucose phosphorylastion, were decreased by $IL-1{\beta}$. EF administration to the mice allowed proportional increasing by stimulation of induction of enzyme activities as high as normal group. These results suggested that EF is highly effective in treatment of diabetes mellitus induce by $IL-1{\beta}$.

청양생진(淸凉生津), 해갈지구(解渴止嘔)의 효능이 있으며, 한국등에서 항당뇨병성 한약으로서 사용되어 온 비파엽의 열수출물의 당뇨병치료효과를 검증하기 위하여 alloxan으로 유발된 당뇨병흰쥐의 혈장포도당농도 및 인슈린농도를 측정하였다. 비파엽처리군의 혈장 포도당농도는 현저히 증가하였으며 혈장인슈린농도는 크게 감소하지 않았다. 한편, 당뇨병유발의 내재적인 요소로 알려진 사이토카인인 $IL-1{\beta}$로 당뇨병을 유발한 뒤 그 효과를 검정했다. 즉, 실험동물을 interleukin-$1{\beta}$로 처리하여 실험적으로 당뇨병을 유발한 후 glucose의 린산화(燐酸化)에 관여하는 glucokinase와 hexokinase의 활성을 살펴보았다. 그 결과, 비파엽엑기스는 interleukin-$1{\beta}$과잉투여 당뇨병으로 인하여 증가된 혈액내glucose농도를 감소시키고 insulin과잉분비를 정상으로 회복시키는데 효과적이었다. 동시에 $IL-1{\beta}$에 의해 유도된 당뇨마우스에 대한 비파엽(批把葉)엑기스 투여(投與)가 췌장glucokinase와 hexokinase의 활성을 촉진하는 것으로 나타나 $IL-1{\beta}$유발 당뇨병에 효과가 있는 것으로 사료되었다.