Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Cloning and Nucleotide Sequence Analysis of the M Protein of a Korean Isolate of Infectious Hematopoietic Necrosis Virus

한국에서 분리된 전염성 조혈괴저바이러스의 M 단백질의 유전자 클로닝과 염기서열 분석

  • Park, Jeong-Min (Department of Biological Science, University of Ulsan) ;
  • Kim, Hyun-Ju (Department of Biological Science, University of Ulsan) ;
  • Mun, Chang-Hoon (Department of Biological Science, University of Ulsan) ;
  • Cho, Wha-Ja (Department of Biological Science, University of Ulsan) ;
  • Cha, Seung-Ju (Department of Biological Science, University of Ulsan) ;
  • Yoon, Won-Joon (Department of Biological Science, University of Ulsan) ;
  • Park, Jeoug-Jae (Department of Biological Science, University of Ulsan) ;
  • Lee, Eun-Hee (Department of Molecular Biology, Pusan National University) ;
  • Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Agency) ;
  • Sohn, Sang-Gyu (Pathology Division, National Fisheries Research and Development Agency) ;
  • Park, Jeong-Woo (Department of Biological Science, University of Ulsan)
  • Received : 1998.03.18
  • Accepted : 1998.05.18
  • Published : 1998.06.01
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Abstract

In order to identify the characteristics of a Korean isol ate of infectious hematopoietic necrosis virus(IHNV), IHNV-PRT, we have cloned and analyzed cDNAs coding for matrix protein M1 and M2 of the IHNV-PRT. The Ml gene contained 693 bp open reading frame and encoded a protein of 230 amino acids with a molecular weight of 25.9 kDa. The M2 gene had 588 bp open reading frame, encoding a protein of 195 amino acids with a molecular weight of 21.9 kDa. On the deduced amino-acid sequences, M1 and M2 of the IHNV-PRT were found to be 92-93% (M1) and 97% (M2) identical to those of foreign isolates of IHNV. These results indicate that M genes of the IHNV are highly conserved among different strains of IHNV.

한국에서 분리된 전염성 조혈괴저바이러스(infectious hematopoietic necrosis virus, IHNV)인 IHNV-PRT의 특성을 규명하기 위하여 IHNV-PRT의 matrix 단백질인 M1 및 M2를 암호화하고 있는 cDNA를 클로닝하여 이들의 염기서열을 분석하였다. M1은 693 bp 크기의 open reading frame을 포함하였으며 이로부터 230개의 아미노산으로 구성된 25.9 kDa의 분자량을 가진 단백질이 합성될 수 있다. M2는 588 bp 크기의 open reading frame을 지니고 있으며 195개의 아미노산으로 구성된 21.9 kDa의 분자량을 지닌 단백질이 합성될 수 있다. IHNV-PRT의 M1과 M2의 아미노산 서열을 외국에서 분리된 IHNV들과 비교 분석한 결과 M1은 92-93%, M2는 97%의 상동성을 보였다. 이러한 사실은 IHNV의 M 단백질 유전자들은 IHNV의 strain에 관계없이 매우 보존되어 있음을 나타내준다.

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Acknowledgement

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