Journal of Preventive Medicine and Public Health
- 제31권4호
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- Pages.680-691
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- 1998
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- 1975-8375(pISSN)
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- 2233-4521(eISSN)
흰쥐와 마우스에서 Ethanol이 요중 트리클로로에틸렌 대사산물 배설량에 미치는 영향
Species Differences in Effect of Ethanol to Urinary Metabolites Excretion of Trichloroethylene in Mice and Rats
- 강은용 (중앙대학교 의과대학 예방의학교실) ;
-
박정덕
(중앙대학교 의과대학 예방의학교실) ;
-
홍연표
(중앙대학교 의과대학 예방의학교실) ;
- 장임원 (중앙대학교 의과대학 예방의학교실)
- Kang, Eun-Yong (Department of Preventive Medicine, College of Medicine, Chungang University) ;
-
Park, Jung-Duck
(Department of Preventive Medicine, College of Medicine, Chungang University) ;
-
Hong, Yeon-Pyo
(Department of Preventive Medicine, College of Medicine, Chungang University) ;
- Chang, Im-Won (Department of Preventive Medicine, College of Medicine, Chungang University)
- 발행 : 1998.12.01
초록
본 연구는 흰쥐와 마우스에서 TCE 경구투여 후 주대사산물인 TCE-OH와 TCA의 요중 배설량과 시간에 따른 배설량의 변화와 이에 대한 ethanol의 영향 및 종간의 차이를 조사하여 다음과 같은 결과를 얻었다. 1. TCE 경구 투여 후 요중 TTC 배설양상은 실험동물의 종간에 현저한 차이가 있었으며, 흰쥐에서는 TTC 배설량이 투여량에 따라 증가하다가 체중 kg당 1,000 mg 용량 이상에서 포화되는 양상을 나타내었으나, 마우스에서는 2,000 mg까지 거의 선형적으로 증가하였다. 투여용량에 따른 TTC 배설 량은 흰쥐보다 마우스에서 유의하게 높았다(p<0.01). 2. TCE 투여 후 경과시간에 따른 대사산물 배설양상은 종이나 투여용량에 관계없이 비슷한 양상을 나타내었다. TCE-OH는 12시간에서 거의 대부분
This study was conducted to examine the species differences in the urinary excretion of trichloroethanol(TCE-OH) and trichloroacetic acid(TCA) of trichloroethylene (TCE) metabolites and the effect of ethanol on these metabolites in mice and rats. TCE administered to Male Sprague Dawley rats and ICR mice as a single oral dose(100, 200, 500, 1,000 or 2,000 mg/kg body weight) and ethanol(3.0 g/kg body weight) was taken orally 12 hours before TCE administration. The metabolites in urine were measured 0, 12, 24, 36 and 48 hours after TCE administration. The results of metabolite excretion were as follows; Total trichlorocompounds(TTC) in urine increased with TCE dose in mice while increased only below dose of 1,000 mg/kg TCE in rats. The net excretion of TCE metabolites was significantly greater in mice than rats, although the proportion of TCE-OH to TCA was not different between mice and rats. These findings indicate that mice were internally exposed to significantly higher concentration of TCE metabolites than rats and this trend appeared to be more prominent with the increase of TCE dose. Ethanol increased significantly TCE-OH in urine of rats while the increase of TCE-OH induced by ethanol was not significant in mice, and didn't increase TCA of urine in both of rats and mice. This result suggests that the effect of ethanol on TCE metabolism may be due to the increase of TCE-OH.