대한수의학회지 (Korean Journal of Veterinary Research)
- 제38권4호
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- Pages.745-750
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- 1998
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
식육중 Escherichia coli O157 검출을 위한 enzyme immunoassay 기법 개발
Development of an enzyme immunoassay for detection of Escherichia coli O157 in meat
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정병열
(농림부 수의과학검역원 세균과) ;
- 정석찬 (농림부 수의과학검역원 세균과) ;
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조동희
(농림부 수의과학검역원 세균과) ;
- 김종염 (농림부 수의과학검역원 세균과) ;
-
박용호
(서울대학교 수의과대학) ;
- 신쌍재 (코넬대학교 수의과대학) ;
- 김성국 (코넬대학교 수의과대학) ;
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김봉환
(경북대학교 수의과대학)
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Jung, Byeong-yeal
(Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
- Jung, Suk-chan (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
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Cho, Dong-hee
(Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
- Kim, Jong-yeom (Department of Bacteriology, National Veterinary Research and Quarantine Services, MAF) ;
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Park, Yong-ho
(College of veterinary Medicine, Seoul National University) ;
- Shin, Sang-jae (New York State College of Veterinary Medicine, Cornell University) ;
- Kim, Sung-guk (New York State College of Veterinary Medicine, Cornell University) ;
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Kim, Bong-hwan
(College of Veterinary Medicine, Kyungpook National University)
- 투고 : 1998.03.25
- 발행 : 1998.12.22
초록
A sensitive and rapid enzyme immunoassay(EIA) to detect Escherichia coli O157 in ground beef was developed by using a sandwich type assay with polyclonal antibodies to E coli O157. E coli O157 in ground beef could be detected within 15hr, including incubation for 12hr in enrichment broth and 3hr in immunoassay. The EIA could detect