한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제31권4호
- /
- Pages.516-522
- /
- 1998
- /
- 0374-8111(pISSN)
- /
- 2287-8815(eISSN)
검정해면으로부터 항균성을 가진 방선균의 분리 동정 및 항균물질의 구조
Identification of an Actinomycetes Strain, MSA-1, Originated from Sponge, Halichondria okadai, and its Antimicrobial Component
- LEE Jong-Soo (Dept. of Marine Food Science and Technology, institute of Marine Industry, Gyeungsang National University) ;
- CHOI Jong-Duck (Dept. of Marine Food Science and Technology, institute of Marine Industry, Gyeungsang National University)
- 발행 : 1998.07.01
초록
검정해면 (Halichondlia okadai)에 공생하는 것으로 추정되는 항균성을 가진 방선균주를 분리하여 SEM, 형태학적, 화학 분류학적 방법에 의하여 균주를 동정하였으며, 이 균주를 marin broth 2216 배지에서 대량 b배양하여 균사체로부터 항균물질을 각종 HPLC에 의하여 분리하고 X선 결정 구조 해석. MS, NMR spectrum에 의하여 구조를 확인하였다. ISP 2 배지에서
An Actinomycetes strain, MSA-1, containing antimicrobial component was isolated from the black sponge, Halichondzia okadai, and was identified to a genus level by morphological and chemotaxonornic methods. The gray colored spores were oval type with smooth surface and formed flexibilis spore chains. The cell wall of this strain was type I containing D-aminopimellic acid (D-DAP) and no specific sugar was detected. Phospholipid of the cell membrane was PII type including phophoethanolamine and the major fatty acids of total lipid were branched anteiso-15 : 0, iso-16 : 0, 16 : 0 and iso-17 ; 0. From these results and other characteristics described in the Bergey's Manual, this strain was identificated as a Streptomyces sp. Meanwhile, 10mg of pale yellow colored antimicreobial component was isolated by HPLC method from the cultured Streptomyces sp. (70g of cryophillized mycellis). By crystallographyc analysis, HIRESMS and NMR assignment, the antimicrobial component produced from the strain MSA-1 was elucidated as the staurosporine (indolo[2,3-a]carbazole alkaloid).
키워드