Acknowledgement
Supported by : 교육부 기초과학연구소
Analytical Science and Technology (분석과학)
- Volume 11 Issue 4
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- Pages.281-291
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- 1998
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
A Study of Analytical Method for Trace Metal Ions in Whole Blood and Urine by Inductively Coupled Plasma-Mass Spectrometry using Solid-Liquid Extraction Technique
유도결합 플라스마-질량분석법과 고체-액체 추출법을 이용한 혈액 및 소변중 미량금속의 분석에 관한 연구
- Lee, Won (Research Institute for Basic Sciences and Department of Chemistry, Kyunghee University) ;
- Hur, Young-Hoe (Research Institute for Basic Sciences and Department of Chemistry, Kyunghee University) ;
- Park, Kyung-Su (Research Institute for Basic Sciences and Department of Chemistry, Kyunghee University)
- Received : 1998.03.11
- Published : 1998.08.01
Abstract
An analytical method for the simultaneous measurement of trace Cu, Sn, and Bi in blood and urine has been investigated by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Microwave oven was used for the pretreatment of blood samples using nitric acid and hydrogen peroxide in a closedvessel digestion system with 1 mL whole blood for 8 minutes. Amberlite IRC-718 resin was used as a solid phase in solid-liquid extraction technique for the removal of matrix interferences such as Na, S, P, and other polyatomic ion species. Detection limits for Cu, Sn, and Bi by this method were 0.000375 ng/mL, 0.000297 ng/mL, and 0.000174 ng/mL, respectively. Recoveries of 99.1% for Cu, 102.5% for Sn, and 98.4% for Bi were obtained for the standard spiked NIST SRM 955a blood sample. The developed method was applied for whole real blood and urine samples.
혈액과 소변중의 미량 Cu, Sn 및 Bi를 유도결합 플라스마 질량분석법으로 동시 분석하였다. 혈액시료 1 mL를 closed-vessel digestion system에 넣고 질산과 과산화수소수를 가한 후 microwave oven에서 8분 동안 전처리하였다. Amberlite IRC-718 수지를 정지상으로 사용한, 고체-액체추출법으로 Na, S, P 및 polyatomic species에 의한 매트릭스 방해를 제거하였다. 위 방법에 대한 검출한계를 구한 결과 Cu는 0.000375 ng/mL, Sn 은 0.000297 ng/mL, Bi는 0.000174 ng/mL 이었다. NIST SRM 955a 혈액시료에 표준용액을 첨가하여 구한 회수율은 Cu의 경우 99.1%, Sn은 102.5%, Bi는 98.4%이었다. 아울러 본 연구에서 조사한 최적기기 및 분석조건에서 실제 혈액과 소변시료의 분석을 시도하였다.