Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Tissue Preparation with t-Butyl Alcohol Freeze-drying Method for Scanning Electron Microscopy: Application for Rat Liver

t-Butyl Alcohol 동결건조법을 이용한 흰쥐 간장의 주사전자현미경적 관찰

  • Uhm, Chang-Sub (Institute of Medical Genetics and Department of Anatomy, Korea University College of Medicine) ;
  • Park, Eun-Kyung (Institute of Medical Genetics and Department of Anatomy, Korea University College of Medicine) ;
  • Park, Chang-Hyun (Electron Microscope Facility, Korea University College of Medicine, Department of Animal Husbandry, College of Veterinary Medicine Konkuk University)
  • 엄창섭 (고려대학교 의과대학 해부학교실 및 유전병연구소) ;
  • 박은경 (고려대학교 의과대학 해부학교실 및 유전병연구소) ;
  • 박창현 (고려대학교 의과대학 전자현미경실, 건국대학교 축산대학 수의학부)
  • Published : 1998.09.01
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Abstract

T-butyl alcohol (TBA) freeze-drying method originally designed by Inoue and Osadake (1989) was adopted to dry specimens for scanning electron microscopy and the results were compared with those dried using critical point dryer (CPD). Small pieces $(1\times1\times3mm)$ of liver of Sprague-Dawley rats were cut and fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer after anesthesia, and processed for scanning electron microscopy by several modifications of TBA freeze-drying methods and by the standard preparation method using CPD. The bile canaliculi and sinusoidal endothelial surface were observed. Tissue dehydrated with TBA before TBA freeze-drying preserved the structures best comparable to those prepared with CPD. This result suggests that combination of dehydration with TBA and TBA freeze-drying is a superior method to the original TBA freeze-drying method dehydrated with ethanol.

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