THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY (대한한방종양학회지)
- Volume 4 Issue 1
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- Pages.131-146
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- 1998
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- 1229-2621(pISSN)
Anti-metastatic Effects of Shiquandabutang on Human Fibrosarcoma cells, HT1080
십전대보탕(十全大補湯)이 암전이(癌轉移) 억제(抑制)에 미치는 영향(影響)
- Yoon, Jae-Ho (Dept. of Oriental Pathology, the College of Oriental Medicine, Kyunghee University) ;
- Choi, Seung-Hoon (Dept. of Oriental Pathology, the College of Oriental Medicine, Kyunghee University) ;
- Ahn, Kyoo-Seok (Dept. of Oriental Pathology, the College of Oriental Medicine, Kyunghee University)
- Published : 1998.12.21
Abstract
To examine the effect of Shiquandabutang on the metastasis of cancer, the following experiments were made. Before the main experiments, the cytotoxicity was measured by putting Shiquandabutang sample in HT1080. Then zymography was made to examine the change of gelatinolytic activity. And western blotting was carried out to examine the changes of Fos, Jun, Ets, the transcription factors of MMP-2, MMP-9, and Erk, JNK on signal transduction pathway to AP-1. Third, in vitro invasion assay with transwells coated by collagen and matrigel was carried out. From the results of the above the following conclusions were obtained. 1. The experimental result about cytotoxicity of Shiquandabutang against HT1080 was as below. The stained cell count after being treated by Shiquandabutang sample