치과방사선 (Journal of Korean Academy of Oral and Maxillofacial Radiology)
- 제28권1호
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- Pages.245-259
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- 1998
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- 1225-049X(pISSN)
DMBA 매식과 방사선 조사로 유도된 백서 타액선 종양에서 H-ras 암유전자의 활성화
ACTIVATION OF H-RAS ONCOGENE IN RAT SALIVARY GLAND TUMORS INDUCED BY DMBA AND IRRADIATION
- 허기수 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
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최종환
(연세대학교 원주의과대학 생화학교실) ;
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최순철
(서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
- 박태원 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소) ;
- 유동수 (서울대학교 치과대학 구강악안면방사선학 교실 및 치학연구소)
- Hu Key-Soon (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University) ;
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Choi Jong-Whan
(Department of Biochemistry, Wonju College of Medicine, Yonsei University) ;
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Choi Soon-Chul
(Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University) ;
- Park Tae-Won (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University) ;
- You Dong-Soo (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University)
- 발행 : 1998.02.01
초록
Cellular transforming genes have been identified in a number of different tumor cell lines and tumor types. A significant number of these oncogenes belong to the ras gene family. The ras gene family consists of three closely related genes:H-ras, K-ras and N-ras which code for a related 21 kDa protein. Mutations in codon 12, 13 and 61 of one of the three ras genes convert these genes into acute oncogenes. The presence of H-ras gene mutations has important prognostic implications in various tumors. Each genomic DNA was isolated from tumors induced by implantation with DMBA, or by treatment with DMBA -implantation/irradiation. When genome DNA was transfected into NIH 3T3 cells and investigated by two-step PCR-RFLP, the fOllowing results were concluded: 1. Transformation foci developed in two groups when the genome DNA of two experimental groups were transfected into NIH 3T3 cells. 2. Transformation efficiency was 0.01-0.02 foci/㎍DNA in the experimental group with the DMBA-implantation, 0.01-0.03 foci/㎍lgDNA in the experimental group with the DMBA-implantation/irradiation according to results of transfection assay. 3. When the point mutation of H-ras gene was investigated by a two-step PCR-RFLP, there was 13.9% (5/36) in the experimental group with the DMBA implantation, 15.4 % (6/39) in the experimental group with the DMBA -implantation/irradiation. 4. The point mutation in codon 12 and 61 of H-ras was 5.6%(2/36) and 8.3%(3/36) in the experimental group with the DMBA implantation. 5. The point mutation in codon 12 and 61 of H-ras gene was 7.7%(3/39) in the experimental group with the DMBA -implantation/irradiation.