Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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In Vitro Skin Irritation Test of Anti-Inflammatory Drugs

소염진통제 약물에 대한 In vitro 피부자극 시험연구

  • Lee, Jong-Kwon (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Dai-Byung (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Eun-Hee (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Sun-Hee (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Ryu, Seung-Rel (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Choi, Ki-Hwan (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Yoon-Jeong (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Pu-Young (Department of Toxicology, National Institute of Toxicological Research, Korea Food and Drug Administration)
  • 이종권 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김대병 (식품의약품안전청 국립독성연구소 독성부) ;
  • 이은희 (식품의약품안전청 국립독성연구소 독성부) ;
  • 이선희 (식품의약품안전청 국립독성연구소 독성부) ;
  • 류승렬 (식품의약품안전청 국립독성연구소 독성부) ;
  • 최기환 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김윤정 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김부영 (식품의약품안전청 국립독성연구소 독성부)
  • Published : 1998.09.01
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Abstract

In vitro skin iritation of anti-inflammatory drugs was investigated in terms of the cytotoxicity method to human skin fibroblast cells. Five anti-inflammatory drugs (Diclofenac, Naproxen, Meclofenamic acid, Ibuprofen and Fnoprofen) which are commercially available as oral preparations or injections were tested. The cytotoxicity of 5 chemicals was evaluated by using MTT[tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. NRU (neutral red uptake) assay and Alamar Blue assay after fibroblast cells had been exposed to the chemicals for 24 hours or 489 hours. The $IC_{50}$ values of the chemicals showed the comparative strength of cytotoxicity as following order of Meclofenamic acid>Diclofenac>Fenoprofen>Ibuprofen>Naproxen. The values of $IC_{50}$ determined by Alamar Blue assay were lower than those of MTT and NRU assay. These data suggest Alamar Blue assay can be useful method for assessing in vitro skin irritation potential of anti-inflammatory drugs.

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