YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Inhibition of Nitric Oxide Synthesis by 8-epi-xanthatin in Activated RAW 264.7 Cells

활성화한 RAW 264.7 세포주에서 8-epi-xanthatin의 Nitric Oxide 생성저해

  • Lee, Hwa-Jin (College of Pharmacy, Research Institute of Pharmaceutical Sciences, Sookmyung Women's University) ;
  • Jeong, Yeon-Su (College of Pharmacy, Research Institute of Pharmaceutical Sciences, Sookmyung Women's University) ;
  • Ryu, Shi-Yong (Korea Research Institute of Chemical Technology) ;
  • Ryu, Jae-Ha (College of Pharmacy, Research Institute of Pharmaceutical Sciences, Sookmyung Women's University)
  • 이화진 (숙명여자대학교 약학대학 약학연구소) ;
  • 정연수 (숙명여자대학교 약학대학 약학연구소) ;
  • 류시용 (한국화학연구소) ;
  • 류재하 (숙명여자대학교 약학대학 약학연구소)
  • Published : 1998.10.01
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Abstract

The nitric oxide (NO) produced in large amounts by inducible nitric oxide synthase is known to be responsible for the vasodilation and hypotension observed in septic shock. We have found that 8-epi-xanthatin from Xanthium strumarium L. inhibited the production of NO in LPS-activated RAW 264.7 cells ($IC_{50}$ value was 1.5 ${\mu}$M). This activity was resulted from the suppressing of inducible nitric oxide synthase enzyme expression.

Keywords

References

  1. Proc. Natl. Acad. Sci. U.S.A. v.84 Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Ignarro, L. J.;Buga, G. M.;Wood, K. S.;Byrns, R. E.;Chaudhuri, G.
  2. Pharmacol. Ther. v.41 Endotheliumderived relaxing factor. Angus, J. A.;Cocks, T. M.
  3. Biochem. Biophys. Res. Commun. v.172 Anti-inflammatory glucocorticoids induction by endotoxin of nitric oxide synthase in the lung, liver and aorta of the rat. Knowles, R. G.;Salter, M.;Brooks, S. L.;Moncada, S.
  4. Biochem. Biophys. Res. Commun. v.173 Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effect on vascular tone : an insight into endotoxic shock. Rees, D. D.;Cellek, S.;Palmer, R. M. J.;Moncada, S.
  5. FASEB J. v.6 Nitric oxide as a secretory product of mammalian cells. Nathan, C.
  6. Biochem. Biophys. Res. Commun. v.208 Nonsteroidal anti-inflammatory drugs inhibit expression of the inducible nitric oxide synthase gene. Aeberhard, E.;Henderson, S. A.;Arabolos, N. S.;Griscavage, J. M.;Castro, F. E.;Barrett, C. T.;Ignarro, L. J.
  7. Proc. Natl. Acad. Sci. U.S.A. v.90 Nitric oxide activates cyclooxygenase enzymes. Salvemini, D.;Misko, T. P.;Masferrer, J. L.;Seibert, K.;Currie, M. G.;Needleman, P.
  8. J. Exp. Med. v.178 Suppression of arthritis by an inhibitor of nitric oxide synthase. McCartney-Francis, N.;Allen, J. B.;Mizel, D. E.;Albina, J. E.;Xie, Q. W.;Nathan, C. F.;Wahl, S. M.
  9. Biochem. J. v.297 Nicotineamide inhibits nitric oxide synthase mRNA induction in activated macrophages. Pellat-Deceunynck, C.;Wietzerbin, J.;Drapier, J. C.
  10. Biochem. J. v.303 Thaloporphine selectively inhibits expression of the inducible, but not the constitutive, nitric oxide synthase. Yu, S. M.
  11. Arch. Pharm. Res. v.21 Yomogin, an inhibitor of nitric oxide production in LPS-activated macrophages. Ryu, J.-H.;Lee, H. J.;Jeong, Y. S.;Ryu, S. Y.;Han, Y. N.
  12. Plalnta Medica A sesquiterpene lactone inhibits the expression of inducible nitric oxide synthase and TNFα in LPS-activated macrophages Lee, H. J.;Kim, N. Y.;Jang, M. K.;Son, H. C.;Kim, K, M.;Sohn, D. H.;Lee, S. H.;Ryu, J.-H.
  13. Natural Product Sciences v.1 Isolation of cytotoxic compounds from the leaves of Xanthium strumarium L. Ahn, J.-W.;No, Z.;Ryu, S. Y.;Zee, O.-P.;Kim, S.-K.
  14. Anal. Biochem. v.126 Analysis of nitrate, nitrite, and $[^{15}N]$ nitrate in biological fluids. Green, L. C.;Wagner, D. A.;Glogowski, J.;Skipper, P. L.;Wishnok, J. S.;Tannenbaum, S. R.