약학회지 (YAKHAK HOEJI)

  • 제42권3호
  • /
  • Pages.336-344
  • /
  • 1998
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

흰쥐 배양 전배자 및 중뇌세포에서 Ochratoxin A의 독성

Embryotoxicity of Ochratoxin A in Cultured Rat Embryonic Midbrain Cells and Whole Embryos

  • 홍진태 (식품의약품안전청 국립독성연구소) ;
  • 박귀례 (식품의약품안전청 국립독성연구소) ;
  • 한순영 (식품의약품안전청 국립독성연구소) ;
  • 박기숙 (식품의약품안전청 국립독성연구소) ;
  • 김형식 (식품의약품안전청 국립독성연구소) ;
  • 오세동 (식품의약품안전청 국립독성연구소) ;
  • 박희정 (식품의약품안전청 국립독성연구소) ;
  • 이이다 (식품의약품안전청 국립독성연구소) ;
  • 장성재 (식품의약품안전청 국립독성연구소)
  • Hong, Jin-Tae (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Park, Kui-Lea (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Han, Soon-Young (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Park, Ki-Sook (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim, Hyung-SIk (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Oh, Se-Dong (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Park, Hee-Jung (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Lee, Rhee-Da (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Jang, Seung-Jae (National Institute of Toxicological Research, Korea Food and Drug Administration)
  • 발행 : 1998.06.01
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초록

Effects of ochratoxin A (OTA) on embryo development were studied in cultured whole embryos from 9.5 day gestation rat for 48 h. OTA (more than $0.5{\mu}g/ml$) induced microcephaly in the cultured rat whole embryos. Protein and DNA content, and DNA synthesis were significantly inhibited by OTA. We next examined whether the microcephaly seen in cultured whole embryo partially results from inhibition of differentiation of embryonic midbrain cells. Embryonic midbrain cells were extracted from 12 day gestation rat embryos, and cultured for 96 hr. OTA ibhibited cell differentiation about 50% over control. We also tested whether OTA-induced embryotoxicity would be associated with oxidative damages. We measured the ${\gamma}$-glutamyltranspeptidase (${\gamma}$-GT) and glutathione peroxidase (GPX) activities, and glutathione (GSH) content in both cultured whole embryos and embryonic midbrain cells. OTA decreased GSH content, whereas slightly increased ${\gamma}$-GT activity, but GPX activity was not significantly changed. These results show that OTA caused the microcephaly and its effect may be partially due to the inhibition of cell differentiation of embryonic midbrain cells, but the role of oxidative damages is not clear in embryotoxicity.

키워드

참고문헌

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