The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 28 Issue 1
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- Pages.71-78
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- 1998
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- 1225-2344(pISSN)
Effective Antitumor Activity of a Recombinant Vaccinia Virus Expressing Murine Interleukin 4
인터루킨-4를 발현하는 재조합 백시니아 바이러스에 의한 암성장의 억제
- Yoon, Kee-Jung (Institute for Molecular Biology and Genetics, Seoul National University) ;
- Jin, Ning-Yi (Research Institute, Changchun University of Agricultural and Animal Sciences) ;
- Kim, Sun-Young (Institute for Molecular Biology and Genetics, Seoul National University)
- Published : 1998.03.30
Abstract
Vaccinia virus is the prototype orthopoxvirus that has been used as a vaccine strain for small pox. This virus has been used to express a variety of cellular and viral genes in mammalian cells at high levels. Interleukin-4 (IL-4) has been found to stimulate the proliferation of T cells and enhance the cytolytic activity of cytotoxic T lymphocytes. To test the immunotherapeutic potential of IL-4 delivered in vivo by poxvirus, a recombinant vaccinia virus expressing the murine IL-4 gene (RVVmIL-4) was constructed. A high level of IL-4 production was confirmed by infecting HeLa cells and measuring IL-4 in cell culture supernatant by ELISA. As a tumor model, two cell lines were used; the murine T leukemic line P388 and the murine breast cancer line TS/A. CDF1 mice were intraperitoneally inoculated with