대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제28권3호
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- Pages.267-274
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- 1998
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- 1225-2344(pISSN)
사람세포거대바이러스 (Human Cytomegalovirus)의 극초기항원-1 (Immediate Early-1, IE-1)에 반응하는 c-jun Promoter의 유전자 지도 분석
Mapping of Human Cytomegalovirus IE1 Responsive Elements in the c-jun Promoter
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박정규
(서울대학교 의과대학 미생물학교실) ;
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한태희
(성균관대학교 의과대학 미생물학 및 면역학교실) ;
- 김대중 (서울대학교 의과대학 미생물학교실) ;
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김진희
(서울대학교 의과대학 미생물학교실) ;
- 황응수 (서울대학교 의과대학 미생물학교실) ;
- 최성배 (서울대학교 의과대학 미생물학교실) ;
- 차창룡 (서울대학교 의과대학 미생물학교실)
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Park, Chung-Gyu
(Department of Microbiology, Seoul National University College of Medicine) ;
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Han, Tae-Hee
(Department of Microbiology and Immunology, Sung Kyun Kwan University College of Medicine) ;
- Kim, Dae-Joong (Department of Microbiology, Seoul National University College of Medicine) ;
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Kim, Jin-Hee
(Department of Microbiology, Seoul National University College of Medicine) ;
- Hwang, Eung-Soo (Department of Microbiology, Seoul National University College of Medicine) ;
- Choi, Sung-Bae (Department of Microbiology, Seoul National University College of Medicine) ;
- Cha, Chang-Yong (Department of Microbiology, Seoul National University College of Medicine)
- 발행 : 1998.09.30
초록
Human cytomegalovirus (HCMV) has the ability to activate the expression of many viral and cellular genes. Among various viral proteins, the immediate early proteins (IE1-72kDa, IE2-86kDa) have been known to be potent transactivators. The product of c-jun proto-oncogene is important in cell activation and differentiation. Here, we tried to find out if the IE could activate the c-jun promoter and also tried to identify the responsible sequence elements in the c-jun activation by IE1-72kDa. We found HCMV IE expression transactivated the c-jun promoter in human embryonal lung fibroblasts (HEL). The activation fold by IE1-72kDa, IE2-86kDa and IE2-55kDa was 23, 35, and 5, respectively. When the expression of each IE was combined, it showed synergism. Expression of (IE1-72kDa + IE2-86kDa) and (IE1-72kDa + IE2-86kDa + IE2-55kDa) resulted in 131 and 162 fold increase, respectively. The c-jun promoter region between -117 and -59 contains binding sites for the transcription factors Spl, CAAT, AP-l like (ATF/CREB), and MEF2. Transient expression assays were performed using various reporter plasmids containing the c-jun promoter-regulatory region linked to the luciferase gene and a plasmid expressing HCMV IE1 gene. Deletional and point mutational analysis showed that the sequence between -225 to -160 and the CTF binding site were involved in the up-regulation of c-jun promoter.