The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 28 Issue 3
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- Pages.233-245
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- 1998
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- 1225-2344(pISSN)
Molecular Cloning and Nucleotide Sequence of the Gene Encoding Fusion(F) Protein of the Thermostable Newcastle Disease Virus Isolated from a Diseased Pheasant
꿩에서 분리된 Newcastle Disease Virus 내열성주 (CBP)의 Fusion(F) 유전자 클론닝과 염기서열 분석
- Chang, Kyung-Soo (College of Veterinary Medicine, Chungnam National University) ;
- Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
- Song, Hee-Jong (College of Veterinary Medicine, Chonbuk National University) ;
- Kim, Kui-Hyun (College of Veterinary Medicine, Chungnam National University) ;
- Park, Jong-Hyeon (Virology Division, National Veterinary Research and Quarantine Services)
- 장경수 (충남대학교 수의과대학) ;
- 전무형 (충남대학교 수의과대학) ;
- 송희종 (전북대학교 수의과대학) ;
- 김귀현 (충남대학교 수의과대학) ;
- 박종현 (국립수의과학검역원 바이러스과)
- Published : 1998.09.30
Abstract
The gene encoding F protein of CBP-1 strain, a heat-stable Newcastle disease virus (NDV) isolated from the diseased pheasants in Korea, was characterized by reverse transcription-polymerase chain reaction (RT-PCR), nucleotide and amino acid sequences. Virus RNA was prepared from the chorioallatoic fluid infected with NDV CBP-1 virus and cDNA was amplified by RT-PCR, cloned and sequenced to analyze. The PCR was sensitive as to detect the virus titer above
Keywords
- Nucleotide sequence analysis;
- F protein gene;
- Thermostable Newcastle disease virus;
- NDV isolate from pheasant