The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Human Immunodeficiency Virus-Infected T Cells Are Selectively Killed by Monoclonal Anti-gp120 Antibody Coupled to Pokeweed Antiviral Protein

섬자리공 유래 항바이러스 단백질과 항체 복합체를 이용한 HIV-1 감염세포의 선택적 제거

  • Kang, Mi-Ran (Institute for Molecular Biology and Genetics, Seoul National University) ;
  • Kim, Yoon-Kyu (Korea Research Institute of Bioscience and Biotechnology) ;
  • Hong, Hyo-Jeong (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Myung-Hwan (Department of Biology, College of Science, Kon Kuk University) ;
  • Shin, Hyung-Sik (Department of Pathology, College of Medicine, Hallym University) ;
  • Kim, Sun-Young (Institute for Molecular Biology and Genetics, Seoul National University)
  • 강미란 (서울대학교 유전공학연구소) ;
  • 김윤구 (서울대학교 생명공학연구소) ;
  • 홍효정 (서울대학교 생명공학연구소) ;
  • 조명환 (건국대학교 생물학과) ;
  • 신형식 (한림대학교 의과대학 병리학교실) ;
  • 김선영 (서울대학교 유전공학연구소)
  • Published : 1998.12.30
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Abstract

A murine monoclonal antibody (mAb) specific for the envelope glycoprotein gp120 of human immunodeficiency virus type-I (HIV -1) was chemically coupled to pokeweed antiviral protein (PAP) from Phytolacca americana. The immunotoxin was purified by FPLC using S200 colum. The purified immunotoxin efficiently bound to HIV-infected T cells as evidenced by fluorescenceactivated cell sorter analysis. The immunotoxin selectively killed human T lymphoid lines infected with $HIV-1_{IIIB}$ at less than 250 pM of the immunotoxin cells, while PAP or mAb alone did not have any significant effect on infected cells. The uninfected control T cell lines were not affected. Human cells infected with HIV-2 or other HIV-1 strains were not killed, suggesting that the killing depends completely on the antibody used for coupling. These in vitro results suggest that the PAP-mAb conjugate may be used to selectively remove cells expressing viral antigens from individuals infected with HIV.

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