대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제28권4호
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- Pages.383-391
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- 1998
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- 1225-2344(pISSN)
섬자리공 유래 항바이러스 단백질과 항체 복합체를 이용한 HIV-1 감염세포의 선택적 제거
Human Immunodeficiency Virus-Infected T Cells Are Selectively Killed by Monoclonal Anti-gp120 Antibody Coupled to Pokeweed Antiviral Protein
-
강미란
(서울대학교 유전공학연구소) ;
- 김윤구 (서울대학교 생명공학연구소) ;
- 홍효정 (서울대학교 생명공학연구소) ;
- 조명환 (건국대학교 생물학과) ;
- 신형식 (한림대학교 의과대학 병리학교실) ;
- 김선영 (서울대학교 유전공학연구소)
-
Kang, Mi-Ran
(Institute for Molecular Biology and Genetics, Seoul National University) ;
- Kim, Yoon-Kyu (Korea Research Institute of Bioscience and Biotechnology) ;
- Hong, Hyo-Jeong (Korea Research Institute of Bioscience and Biotechnology) ;
- Cho, Myung-Hwan (Department of Biology, College of Science, Kon Kuk University) ;
- Shin, Hyung-Sik (Department of Pathology, College of Medicine, Hallym University) ;
- Kim, Sun-Young (Institute for Molecular Biology and Genetics, Seoul National University)
- 발행 : 1998.12.30
초록
A murine monoclonal antibody (mAb) specific for the envelope glycoprotein gp120 of human immunodeficiency virus type-I (HIV -1) was chemically coupled to pokeweed antiviral protein (PAP) from Phytolacca americana. The immunotoxin was purified by FPLC using S200 colum. The purified immunotoxin efficiently bound to HIV-infected T cells as evidenced by fluorescenceactivated cell sorter analysis. The immunotoxin selectively killed human T lymphoid lines infected with