The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Sequence and Phylogenetic Analyses of the M and S Genomic Segments of Hantaan and Seoul Viruses

한타바이러스와 서울바이러스의 M 및 S 분절의 염기서열 및 계통분석

  • Song, Ki-Joon (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Yang, Jeong-In (Graduate School, Korea University) ;
  • Kim, Sang-Hyun (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Kim, Jong-Hun (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Lee, Young-Eun (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Baek, Luck-Ju (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Lee, Yong-Ju (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Song, Jin-Won (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University)
  • 송기준 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 양정인 (고려대학교 대학원) ;
  • 김상현 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 김종현 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 이영은 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 백락주 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 이용주 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 송진원 (고려대학교 의과대학 미생물학교실, 바이러스병연구소)
  • Published : 1998.12.30
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Abstract

Hantaan (HTN) and Seoul (SEO) viruses, murid rodent-borne hantaviruses, are known to causes hemorrhagic fever with renal syndrome (HFRS) in Korea. To determine the genomic diversity and molecular phylogeny of HTN and SEO viruses found in Korea, we amplified for part of M and S genomic segments of hantaviruses from sera of HFRS patients and lung tissues of hantavirus seropositive striped-field mice. Both M and S segment of 16 HTN and 2 SEO viruses were amplified by nested reverse transcription-polymerase chain reaction. Based on 324 nucleotides in the M genomic segment, the HTN and SEO strains showed $93.8{\sim}100%$ and $99.1{\sim}99.4%$ homologies, respectively. Similarly, based on 230 nucleotides in the S genomic segment, HTN and SEO strains showed $90.9{\sim}100%$ and 100% homologies, respectively. Phylogenetic analysis of M and S segments indicated that HTN strains could be divided into at least two main groups in M and S trees and the sequence differences detected among the Sand M genomic segments of HTN viruses are consistent with reassortment having taken place between HTN virus strains.

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