Clinical and Experimental Reproductive Medicine
- Volume 25 Issue 1
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- Pages.65-70
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- 1998
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
The Effects of Glutamine on Blastulation of Human Embryos on Vero Cells In Vitro
체세포의 공배양체계에서 단일 에너지원이 인간 배반포기 배의 형성에 미치는 영향
- Park, Kee-Sang (Department of Aminal Science, Taegu University) ;
- Choi, In-Kyung (Department of Aminal Science, Taegu University) ;
- Lee, Jin-Shik (Infertility Medical Center, Han-Mee Women's Clinic) ;
- Song, Hai-Bum (Department of Aminal Science, Taegu University)
- Published : 1998.03.30
Abstract
This study was conducted to investigate the effects of Tissue Culture Medium 199 (TCM) and Dulecco's Modified Eagle Medium (DMEM) on the blastulation and grade of human oocytes on Vero cells in vitro. A cohort of 79 and 93 oocytes in metaphase II stage were used in TCM 199 and DMEM respectively. No differences were found in the numer of oocytes showing two-pronuclei between TCM (82.3%) and DMEM (86.0%). The number of fertilized oocytes reaching the blastocyst was not significant in TCM (60.0%) and DMEM (63.1%). A total of 89 blastocysts were categorized into the four grades (BG1, BG2, BG3 and early) depending on their morphology. The number of embryos achieving the blastocyst grade 1 (BG1) was significantly higher (p<0.05) in DMEM (50.8%) than TCM (15.0%). It is concluded that cultured oocytes in DMEM with glutamine on Vero cells should be significantly increased BG1.
Keywords
- Tissue Culture Medium 199 (TCM);
- Dulbecco's Modified Eagle Medium (DMEM);
- Two-pronuclei;
- Blastulation;
- Blastocyst;
- Blastocyst grade 1 (BG1)