Nitric Oxide가 인간 정자세포의 기능에 미치는 효과에 대한 연구

The Dose-Dependent Effects of Nitric Oxide on Human Sperm Cell Function

  • 주보선 (문화병원 불임연구실) ;
  • 문화숙 (문화병원 불임연구실) ;
  • 박수진 (문화병원 불임연구실) ;
  • 문재연 (문화병원 불임연구실) ;
  • 조재동 (조재동 산부인과) ;
  • 김한도 (부산대학교 자연과학대학 분자생물학과)
  • Joo, Bo-Sun (Infertility Research Laboratory, Moon Hwa Hospital) ;
  • Moon, Hwa-Sook (Infertility Research Laboratory, Moon Hwa Hospital) ;
  • Park, Sue-Jin (Infertility Research Laboratory, Moon Hwa Hospital) ;
  • Moon, Jae-Yeoun (Infertility Research Laboratory, Moon Hwa Hospital) ;
  • Cho, Jae-Dong (Cho Jae Dong OB/GY) ;
  • Kim, Han-Do (Department of Molecular Biology, College of Natural Science, Pusan University)
  • 발행 : 1998.03.30

초록

This study was performed to determine the effects of nitric oxide on human sperm cell function. Semen samples were obtained from normal healthy volunteers. Motile spermatozoas collected by swim-up method were incubated up to 24 hours in Ham's F-10 medium supplemented with a various concentration of sodium nitroprusside (nitric oxide releasing agent). Sperm motility, hyperactivation, acrosome reaction rate, and acrosin activity were determined. The results are as follows; 1. 1mM of SNP resulted in a significant decrease in sperm motility ($44.8%{\pm}8.9%:78.1%{\pm}6.3%$, and hyperactivation $(10.4%{\pm}6.4%:47.7%:{\pm}9.5%)$ after incubation for 3 hours compared with the control group (Ham's F-10 alone), but had no effect on acrosome reaction. 2. At $100{\mu}M$ SNP, sperm motility was reduced after incubation for 6 hours $(54.8%{\pm}3.2%)$ compared with that of the control group $(82.7%{\pm}8.9%)$, but hyperactivation and acrosome reaction were not affected. 3. However, a lower concentration (less than $10{\mu}M$) of SNP had no effect on sperm motility and hyperactivation for 8 hours of incubation but significantly decreased them when incubation periods were increased up to 24 hours compared with the control group. On the other hand, $1{\mu}M$ and $10{\mu}M$ SNP significantly increased the acrosome reaction rate in both acrosomal status ($17.3%{\pm}5.2%$, $23.5%{\pm}4.7%$, respectively) and acrosin activity ($34.3{\mu}IU{\pm}10.5{\mu}IU,\;45.6{\mu}IU{\pm}5.6{\mu}IU$, respectively) as compared with the control group $(7.0%{\pm}4.0%,\;9.5{\mu}IU{\pm}3.4{\mu}IU)$. These results indicate that SNP, NO releasing agent, has a dose-dependent effects on the sperm cell function. Therefore it may positively affect the fertilization by promoting acrosomal reaction at a lower concentration (less than $10{\mu}M$).

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