Clinical and Experimental Reproductive Medicine
- Volume 25 Issue 3
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- Pages.261-268
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- 1998
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
The Effect of Equilibration Temperature and Exposure Time on the Ultrarapid Freezing of 1-cell Mouse Zygote
생쥐 1-세포기배의 초급속 동결에 있어서 평형 온도와 노출시간의 영향
- Chung, Duk-Soo (Infertility Clinic, Hyun Dae Obstetrics and Gynecology) ;
- Kim, Hyung-Kuk (Infertility Clinic, Hyun Dae Obstetrics and Gynecology) ;
- Park, In-Kook (Department of Applied Biology, Dongguk University)
- Published : 1998.09.30
Abstract
The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to playa key role in the development of the embryo. In general, the development of the embryo was more effective at