Journal of Environmental Science International (한국환경과학회지)
- Volume 7 Issue 6
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- Pages.853-857
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- 1998
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- 1225-4517(pISSN)
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- 2287-3503(eISSN)
Purification of Isocitrate lyase Produced from Microbacterium laevaniformans
Microbacterium laevaniformans가 생성하는 Isocitrate lyase의 정제
Abstract
Purification of the isocitrate lyase extracted from Microbacterium laevaniformans was investigated. The isocitrate lyase was purified 43.6 folds by the following continuous treatment with ammonium sulfate fraction, DEAE-cellulose, DEAE-sephacel and Sephadex G-200 chromatography. The purified isocitrate lyase was showed to be a single protein band by polyacrylamide gel electrophoresis. The molecular weight of the purified isocitrate lyase was estimated 54,000 Da by the SDS-polyacrylamide gel electrophoresis. The Km and Vmax values for isocitrate were estimated to be 0.83mM and 0.33units/ml, respectively. Activity of isocitrate lyase was inhibited by cystein-HCl and glutathione.